Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4188306..4188960 Replicon chromosome
Accession NZ_CP043950
Organism Escherichia coli strain ST95-32

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag F3J96_RS20890 Protein ID WP_021515140.1
Coordinates 4188306..4188713 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag F3J96_RS20895 Protein ID WP_000354046.1
Coordinates 4188694..4188960 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3J96_RS20870 4184263..4185996 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
F3J96_RS20875 4186002..4186712 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
F3J96_RS20880 4186737..4187633 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
F3J96_RS20885 4187745..4188266 + 522 WP_001055874.1 flavodoxin FldB -
F3J96_RS20890 4188306..4188713 - 408 WP_021515140.1 protein YgfX Toxin
F3J96_RS20895 4188694..4188960 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
F3J96_RS20900 4189203..4190183 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
F3J96_RS20905 4190260..4190919 - 660 WP_000250274.1 hemolysin III family protein -
F3J96_RS20910 4191083..4191394 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
F3J96_RS20915 4191439..4192872 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16008.94 Da        Isoelectric Point: 10.9373

>T139652 WP_021515140.1 NZ_CP043950:c4188713-4188306 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLCWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T139652 NZ_CP058771:788942-789238 [Shigella flexneri]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT139652 WP_000354046.1 NZ_CP043950:c4188960-4188694 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT139652 NZ_CP058771:789240-789635 [Shigella flexneri]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References