Detailed information of TA system TA139574

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26006..26275
• Replicon: plasmid pMPCMY-2
• Accession: NZ_CP043945
• Organism: Escherichia coli strain AR216.2b

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: F3P14_RS25065
• Protein ID: WP_001312861.1
• Coordinates: 26117..26275 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26006..26071 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P14_RS25040	21716..22243	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
F3P14_RS25045	22301..22534	+	234	WP_000006003.1	DUF905 family protein	-
F3P14_RS25050	22595..24618	+	2024	Protein_33	ParB/RepB/Spo0J family partition protein	-
F3P14_RS25055	24687..25121	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
F3P14_RS25060	25118..25837	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	25849..26073	+	225	NuclAT_0	-	-
-	25849..26073	+	225	NuclAT_0	-	-
-	25849..26073	+	225	NuclAT_0	-	-
-	25849..26073	+	225	NuclAT_0	-	-
-	25849..26073	-	225	NuclAT_0	-	-
F3P14_RS25530	25858..26037	-	180	WP_001309233.1	hypothetical protein	-
-	26006..26071	+	66	NuclAT_1	-	Antitoxin
F3P14_RS25065	26117..26275	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
F3P14_RS25535	26513..26890	-	378	Protein_38	hypothetical protein	-
F3P14_RS25085	27190..27486	+	297	WP_001272251.1	hypothetical protein	-
F3P14_RS25090	27597..28418	+	822	WP_001234445.1	DUF945 domain-containing protein	-
F3P14_RS25095	28715..29317	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
F3P14_RS25105	29640..30023	+	384	WP_001354030.1	relaxosome protein TraM	-
F3P14_RS25110	30217..30888	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
F3P14_RS25115	31025..31252	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / blaCMY-2	-	1..66573	66573

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T139574 WP_001312861.1 NZ_CP043945:26117-26275 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T139574 NZ_CP058743:730895-731380 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT139574 NZ_CP043945:26006-26071 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin