Detailed information of TA system TA139339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 121023..121780
• Replicon: plasmid p1RM8082
• Accession: NZ_CP043829
• Organism: Escherichia coli strain RM8082

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A136WTZ1
• Locus tag: F1745_RS27495
• Protein ID: WP_000501973.1
• Coordinates: 121295..121780 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: F1745_RS27490
• Protein ID: WP_001352814.1
• Coordinates: 121023..121307 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1745_RS27470 (117302)	117302..117644	+	343	Protein_122	IS3 family transposase	-
F1745_RS27475 (117700)	117700..117855	+	156	Protein_123	transposase domain-containing protein	-
F1745_RS27480 (118093)	118093..119040	+	948	WP_032215132.1	omptin family outer membrane protease OmpP	-
F1745_RS27485 (119133)	119133..120632	+	1500	WP_032215133.1	alpha/beta hydrolase	-
F1745_RS27490 (121023)	121023..121307	+	285	WP_001352814.1	DUF1778 domain-containing protein	Antitoxin
F1745_RS27495 (121295)	121295..121780	+	486	WP_000501973.1	GNAT family N-acetyltransferase	Toxin
F1745_RS27500 (121870)	121870..123198	+	1329	WP_047088448.1	IS4-like element IS4 family transposase	-
F1745_RS27505 (123516)	123516..124538	-	1023	WP_032215002.1	IS110 family transposase	-
F1745_RS27510 (125274)	125274..125477	+	204	Protein_130	transposase	-
F1745_RS27515 (125641)	125641..126279	-	639	WP_032215003.1	ParB N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyB / hlyD	1..146074	146074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-93)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17656.44 Da
• Isoelectric Point: 9.6133

>T139339 WP_000501973.1 NZ_CP043829:121295-121780 [Escherichia coli]
MGCVTAPEPLSSFHQVAEFVSGETVLDDWLKQKGLKNQALGATRTFVVCRKGTQQVVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDASFRGKGLGADLLHDAVCRCYRVAENIGVRAIMVHALTENAKHFYIHHGFKPSKTQVQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T139339 NZ_CP043829:121295-121780 [Escherichia coli]
ATGGGATGTGTAACAGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAACTGTTCTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCTCTCGGAGCCACCCGGACTTTTGTTGTATGCAGGAAAGGTACGC
AACAAGTAGTTGGTTTTTACTCACTGGCTACTGGCAGTGTCAACCATACAGAAGCTACAGGCAACCTTCGGCGCAATATG
CCTGACCCTATCCCTGTCATTATACTTGCACGCCTGGCTGTTGATGCGTCATTCCGTGGTAAAGGGCTTGGTGCAGATTT
ACTGCATGATGCGGTTTGTCGTTGTTACAGAGTGGCCGAAAATATAGGTGTGCGTGCAATTATGGTTCATGCACTTACGG
AAAACGCAAAACACTTTTATATTCACCATGGTTTTAAGCCATCAAAAACCCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10915.58 Da
• Isoelectric Point: 4.6926

>AT139339 WP_001352814.1 NZ_CP043829:121023-121307 [Escherichia coli]
MIYGGFMKSGVQLNLRARESQRILIDTAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADEP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT139339 NZ_CP058651:1109186-1109605 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A136WTZ1