Detailed information of TA system TA139148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5126654..5127279
• Replicon: chromosome
• Accession: NZ_CP043597
• Organism: Klebsiella pneumoniae strain WCHKP085031

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B7D33_RS25180
• Protein ID: WP_094645114.1
• Coordinates: 5126654..5127037 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: B7D33_RS25185
• Protein ID: WP_004150355.1
• Coordinates: 5127037..5127279 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D33_RS25165 (5124020)	5124020..5124922	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
B7D33_RS25170 (5124919)	5124919..5125554	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
B7D33_RS25175 (5125551)	5125551..5126480	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
B7D33_RS25180 (5126654)	5126654..5127037	-	384	WP_094645114.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7D33_RS25185 (5127037)	5127037..5127279	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
B7D33_RS25190 (5127484)	5127484..5128401	+	918	WP_088497967.1	alpha/beta hydrolase	-
B7D33_RS25195 (5128415)	5128415..5129356	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
B7D33_RS25200 (5129401)	5129401..5129838	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
B7D33_RS25205 (5129835)	5129835..5130695	-	861	WP_002882807.1	virulence factor BrkB family protein	-
B7D33_RS25210 (5130689)	5130689..5131288	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14324.57 Da
• Isoelectric Point: 6.7186

>T139148 WP_094645114.1 NZ_CP043597:c5127037-5126654 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGCREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T139148 NZ_CP058410:c172752-172498 [Trichlorobacter lovleyi]
ATGATGATAAAATTCTCAACCGCAGCATGGGAGGACTATCTCTACTGGCAGACTACCGACAAGGCGATGCTGAAACGGAT
CAATCAACTTATCCGGGATATCCAGCGAGAACCGTTTGCCGGTATCGGCAAGCCGGAACCGCTCAAACATCAATTGGCCG
GTTTCTGGTCACGCAGAATAGACAGCTGCCACCGCTTGGTCTATGCGGTTGAAGGTGATACGCTCCTGATTGCACAGTGT
AGAGATCATTATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT139148 WP_004150355.1 NZ_CP043597:c5127279-5127037 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT139148 NZ_CP058410:c172991-172749 [Trichlorobacter lovleyi]
CTGACCTATACCTATACGCGCCAGCATTTTGCAGAGGTCATGAAATCAGTAAATGAAGACCACGTTCCCGTGGTGGTGAC
CAGCCAGCGCGGCAAACCGGTTGTCATCCTTTCCCTCGACGATTTCCACGCTTATGAAGAAACCGCCTATCTGCTCCGTA
ATCCCCAAGGGGCCAAACGGTTGCTTGAATCTGTGGAGGAACTGCGTGCAGGCGGAGGGCAGACCAGAGACCTGACGGAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9