Detailed information of TA system TA139087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3889656..3890253
• Replicon: chromosome
• Accession: NZ_CP043585
• Organism: Klebsiella pneumoniae subsp. ozaenae strain SCKP020151

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: CLQ49_RS18865
• Protein ID: WP_004893639.1
• Coordinates: 3889936..3890253 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: CLQ49_RS18860
• Protein ID: WP_004142561.1
• Coordinates: 3889656..3889943 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ49_RS18830 (CLQ49_18950)	3885736..3885984	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
CLQ49_RS18835 (CLQ49_18955)	3886002..3886343	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
CLQ49_RS18840 (CLQ49_18960)	3886374..3887489	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
CLQ49_RS18845 (CLQ49_18965)	3887669..3888250	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
CLQ49_RS18850 (CLQ49_18970)	3888250..3888618	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
CLQ49_RS18855 (CLQ49_18975)	3888738..3889391	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
CLQ49_RS18860 (CLQ49_18980)	3889656..3889943	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
CLQ49_RS18865 (CLQ49_18985)	3889936..3890253	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLQ49_RS18870 (CLQ49_18990)	3890438..3891481	-	1044	WP_004893645.1	DUF2157 domain-containing protein	-
CLQ49_RS18875 (CLQ49_18995)	3892147..3893013	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
CLQ49_RS18880 (CLQ49_19000)	3893122..3894549	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T139087 WP_004893639.1 NZ_CP043585:c3890253-3889936 [Klebsiella pneumoniae subsp. ozaenae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T139087 NZ_CP058353:c3788655-3788266 [Serratia marcescens]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTGCGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCCGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATGGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT139087 WP_004142561.1 NZ_CP043585:c3889943-3889656 [Klebsiella pneumoniae subsp. ozaenae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT139087 NZ_CP058353:c3788922-3788659 [Serratia marcescens]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAATTTCTTGGCGCGGAAGATCGTCAGCAAGGGGTGGCAGACCGC
GATCCGTTCTCAGAGGAAGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1