Detailed information of TA system TA138705

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 88467..88893
• Replicon: plasmid pNMBU-W13E19_01
• Accession: NZ_CP043407
• Organism: Escherichia coli strain NMBU-W13E19

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FZN32_RS25240
• Protein ID: WP_001312861.1
• Coordinates: 88467..88625 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	88669..88893 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN32_RS25200	83579..83806	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
FZN32_RS25205	83894..84571	-	678	WP_001348626.1	PAS domain-containing protein	-
FZN32_RS25210	84705..85088	-	384	WP_001151566.1	relaxosome protein TraM	-
FZN32_RS25215	85419..86021	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
FZN32_RS25220	86318..87139	-	822	WP_001234469.1	DUF945 domain-containing protein	-
FZN32_RS25225	87258..87545	-	288	WP_000107535.1	hypothetical protein	-
FZN32_RS25230	87570..87776	-	207	WP_000275859.1	hypothetical protein	-
FZN32_RS25235	87778..87966	-	189	WP_032143370.1	hypothetical protein	-
FZN32_RS25240	88467..88625	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	88669..88893	-	225	NuclAT_0	-	Antitoxin
-	88669..88893	-	225	NuclAT_0	-	Antitoxin
-	88669..88893	-	225	NuclAT_0	-	Antitoxin
-	88669..88893	-	225	NuclAT_0	-	Antitoxin
FZN32_RS25245	88705..88893	+	189	WP_001299721.1	hypothetical protein	-
FZN32_RS25250	88905..89624	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
FZN32_RS25255	89621..90055	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
FZN32_RS25260	90110..92068	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
FZN32_RS25265	92134..92367	-	234	WP_000005990.1	DUF905 family protein	-
FZN32_RS25270	92430..92969	-	540	WP_000290841.1	single-stranded DNA-binding protein	-
FZN32_RS25275	93203..93391	-	189	WP_032143370.1	hypothetical protein	-
FZN32_RS25280	93612..93842	-	231	WP_021526513.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA14 / mph(A)	-	1..122112	122112

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T138705 WP_001312861.1 NZ_CP043407:c88625-88467 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T138705 NZ_CP043407:c88625-88467 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT138705 NZ_CP043407:c88893-88669 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin