Detailed information of TA system TA138536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 16720..17363
• Replicon: plasmid pCTXM15_085104
• Accession: NZ_CP043364
• Organism: Klebsiella pneumoniae strain WCHKP085104

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A7D6UII7
• Locus tag: B7D52_RS25885
• Protein ID: WP_032425563.1
• Coordinates: 16947..17363 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: B7D52_RS25880
• Protein ID: WP_001261276.1
• Coordinates: 16720..16950 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D52_RS25840	12258..12371	+	114	WP_014343462.1	hypothetical protein	-
B7D52_RS25845 (B7D52_26105)	12500..12757	+	258	WP_009310077.1	hypothetical protein	-
B7D52_RS25850 (B7D52_26110)	12815..13594	-	780	WP_023287113.1	site-specific integrase	-
B7D52_RS25855 (B7D52_26115)	13792..14808	-	1017	WP_009309921.1	hypothetical protein	-
B7D52_RS25860 (B7D52_26120)	14842..15177	-	336	WP_009309920.1	hypothetical protein	-
B7D52_RS25865	15227..15367	-	141	WP_162898808.1	hypothetical protein	-
B7D52_RS25870 (B7D52_26125)	15596..15901	-	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	-
B7D52_RS25875 (B7D52_26130)	15903..16121	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	-
B7D52_RS25880 (B7D52_26145)	16720..16950	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B7D52_RS25885 (B7D52_26150)	16947..17363	+	417	WP_032425563.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7D52_RS25890 (B7D52_26155)	17404..18282	-	879	WP_064182004.1	restriction endonuclease	-
B7D52_RS25895 (B7D52_26160)	18944..20221	+	1278	WP_009309915.1	HlyD family secretion protein	-
B7D52_RS25900 (B7D52_26165)	20211..22319	+	2109	WP_032427226.1	peptidase domain-containing ABC transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-15 / blaTEM-1C	-	1..127885	127885

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.56 Da
• Isoelectric Point: 8.5403

>T138536 WP_032425563.1 NZ_CP043364:16947-17363 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T138536 NZ_CP058147:c32069-31659 [Enterobacter hormaechei]
GTGAACAAAATGTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATCACCTGGGCCGAAGTGTCACTGGCATCACAGGAAG
CAGGACCAGCCGCACAGAAGCTGGCTGACGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGACCGTACCGCGGTG
GACGCGACCACGGAAATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGGCCGAACGACACGGCGATCGCCGGGCA
CGCCATCGCCGCCGGTGCCGTGCTGGTGACCAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTTGTGCTGGAAGACT
GGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT138536 WP_001261276.1 NZ_CP043364:16720-16950 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT138536 NZ_CP058147:c32296-32066 [Enterobacter hormaechei]
ATGAGAACCGTATCCATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A7D6UII7

Antitoxin

• AlphaFold DB: A0A387K023