Detailed information of TA system TA137683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 3796175..3797612
• Replicon: chromosome
• Accession: NZ_CP043199
• Organism: Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: FYA11_RS18475
• Protein ID: WP_000155570.1
• Coordinates: 3796175..3797248 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: FYA11_RS18480
• Protein ID: WP_000461704.1
• Coordinates: 3797241..3797612 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA11_RS18460	3791608..3793797	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
FYA11_RS18465	3793794..3795410	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
FYA11_RS18470	3795770..3796033	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
FYA11_RS18475	3796175..3797248	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
FYA11_RS18480	3797241..3797612	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
FYA11_RS18485	3797967..3798830	+	864	WP_000787597.1	GTPase family protein	-
FYA11_RS18490	3798922..3799743	+	822	WP_000197391.1	DUF945 domain-containing protein	-
FYA11_RS18495	3799960..3800661	+	702	WP_000189409.1	WYL domain-containing protein	-
FYA11_RS18500	3800702..3800938	+	237	WP_001144029.1	hypothetical protein	-
FYA11_RS18505	3800938..3801381	+	444	WP_000824222.1	hypothetical protein	-
FYA11_RS18510	3801405..3801872	+	468	WP_000702495.1	hypothetical protein	-
FYA11_RS18515	3802097..3802411	-	315	WP_000065803.1	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T137683 WP_000155570.1 NZ_CP043199:3796175-3797248 [Escherichia coli O16:H48]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T137683 NZ_CP056896:c4921816-4921442 [Citrobacter sp. RHBSTW-00021]
ATGGCCACAGGATCTGCGCTTTTTGATACCAACATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAACGCAATCAGCCTCATTACATGGATGGAGTTGATGGTGGGCGCAAAAAAATATCATCAGG
AATCTCGCACACGCGTTGCGCTAGGCGCTTTTAATGTGATAGGTGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATCATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGCG
TAATACCAGGGATTTTGCAGGCATATCCGGCGTGGTCACACCGTATACGTTATAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT137683 WP_000461704.1 NZ_CP043199:3797241-3797612 [Escherichia coli O16:H48]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT137683 NZ_CP056896:c4922058-4921816 [Citrobacter sp. RHBSTW-00021]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGAAGTGATCAAACGTCTTGATGATCTCAAGGT
GCAGCGTAATCTGCCGCGGGCCGAGCTTTTGCGGGAGGCTGTTGAACAGTATCTTGAGAGGCAGGATCAAGCTGAAACGA
CAATTTCACGTGCTCTTGGGCTATGGCAAGGTTGTGAGGAAGATGGCGTTGATTATCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4