Detailed information of TA system TA137609

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4247849..4248261
• Replicon: chromosome
• Accession: NZ_CP043195
• Organism: Escherichia coli O16:H48 strain PG20180062

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: FYA13_RS20770
• Protein ID: WP_000132601.1
• Coordinates: 4247920..4248261 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4247849..4247925 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA13_RS20760	4244712..4246301	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
FYA13_RS20765	4246298..4247692	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
-	4247849..4247925	-	77	NuclAT_14	-	Antitoxin
-	4247849..4247925	-	77	NuclAT_14	-	Antitoxin
-	4247849..4247925	-	77	NuclAT_14	-	Antitoxin
-	4247849..4247925	-	77	NuclAT_14	-	Antitoxin
-	4247849..4247925	-	77	NuclAT_15	-	Antitoxin
-	4247849..4247925	-	77	NuclAT_15	-	Antitoxin
-	4247849..4247925	-	77	NuclAT_15	-	Antitoxin
-	4247849..4247925	-	77	NuclAT_15	-	Antitoxin
FYA13_RS20770	4247920..4248261	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
FYA13_RS20775	4248423..4249802	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
FYA13_RS20780	4249802..4250848	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T137609 WP_000132601.1 NZ_CP043195:4247920-4248261 [Escherichia coli O16:H48]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T137609 NZ_CP043195:4247920-4248261 [Escherichia coli O16:H48]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT137609 NZ_CP043195:c4247925-4247849 [Escherichia coli O16:H48]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin