Detailed information of TA system TA136951

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 69210..70045
• Replicon: chromosome
• Accession: NZ_CP043033
• Organism: Escherichia coli strain XDL

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4TIK8
• Locus tag: EOV66_RS00305
• Protein ID: WP_000854694.1
• Coordinates: 69210..69587 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8S7S1S5
• Locus tag: EOV66_RS00310
• Protein ID: WP_065793167.1
• Coordinates: 69677..70045 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV66_RS00275	64869..65792	-	924	WP_000535950.1	carboxylate/amino acid/amine transporter	-
EOV66_RS00280	65903..67087	-	1185	WP_001172866.1	sugar efflux transporter	-
EOV66_RS00285	67469..67615	-	147	WP_001328030.1	hypothetical protein	-
EOV66_RS00290	67864..68706	-	843	WP_001696593.1	DUF4942 domain-containing protein	-
EOV66_RS00295	68791..68988	-	198	WP_086795284.1	DUF957 domain-containing protein	-
EOV66_RS00300	69064..69213	-	150	Protein_58	hypothetical protein	-
EOV66_RS00305	69210..69587	-	378	WP_000854694.1	TA system toxin CbtA family protein	Toxin
EOV66_RS00310	69677..70045	-	369	WP_065793167.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
EOV66_RS00315	70208..70429	-	222	WP_000692303.1	DUF987 domain-containing protein	-
EOV66_RS00320	70492..70968	-	477	WP_001186710.1	RadC family protein	-
EOV66_RS00325	70980..71459	-	480	WP_072742953.1	antirestriction protein	-
EOV66_RS00330	71541..72359	-	819	WP_001175149.1	DUF945 domain-containing protein	-
EOV66_RS00335	72449..72682	-	234	WP_001278287.1	DUF905 family protein	-
EOV66_RS00340	72688..73365	-	678	WP_001097305.1	hypothetical protein	-
EOV66_RS00345	73513..74193	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14008.02 Da
• Isoelectric Point: 7.7293

>T136951 WP_000854694.1 NZ_CP043033:c69587-69210 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T136951 NZ_CP043033:c69587-69210 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13555.50 Da
• Isoelectric Point: 6.6249

>AT136951 WP_065793167.1 NZ_CP043033:c70045-69677 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT136951 NZ_CP056372:331938-332159 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCTGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A1M2F934