Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1975194..1975744 Replicon chromosome
Accession NZ_CP042946
Organism Escherichia fergusonii strain ATCC 35470

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag ABH49_RS09475 Protein ID WP_001160963.1
Coordinates 1975194..1975508 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag ABH49_RS09480 Protein ID WP_000125566.1
Coordinates 1975511..1975744 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABH49_RS09450 1970869..1972155 + 1287 WP_000800490.1 peptidylprolyl isomerase SurA -
ABH49_RS09455 1972155..1973144 + 990 WP_105284067.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
ABH49_RS09460 1973141..1973962 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
ABH49_RS09465 1973965..1974342 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
ABH49_RS09470 1974349..1975197 + 849 WP_088541456.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
ABH49_RS09475 1975194..1975508 - 315 WP_001160963.1 CcdB family protein Toxin
ABH49_RS09480 1975511..1975744 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ABH49_RS09485 1975830..1976309 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
ABH49_RS09490 1976502..1978364 - 1863 WP_000377102.1 glutathione-regulated potassium-efflux system protein KefC -
ABH49_RS09495 1978357..1978887 - 531 WP_032183187.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
ABH49_RS09500 1978995..1980326 - 1332 WP_001183184.1 MFS transporter -
ABH49_RS09505 1980385..1980672 - 288 WP_105284066.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T136721 WP_001160963.1 NZ_CP042946:c1975508-1975194 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T136721 NZ_CP042946:c1975508-1975194 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT136721 WP_000125566.1 NZ_CP042946:c1975744-1975511 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT136721 NZ_CP042946:c1975744-1975511 [Escherichia fergusonii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References