Detailed information of TA system TA136420

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51771..52040
• Replicon: plasmid pNMBU-W12E19_01
• Accession: NZ_CP042885
• Organism: Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: FVP48_RS00305
• Protein ID: WP_001302184.1
• Coordinates: 51882..52040 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	51771..51836 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVP48_RS00275	46857..47534	+	678	WP_001401776.1	IS66 family insertion sequence hypothetical protein	-
FVP48_RS00280	47531..47878	+	348	WP_000631709.1	IS66 family insertion sequence element accessory protein TnpB	-
FVP48_RS00285	47898..49499	+	1602	WP_032180886.1	IS66 family transposase	-
FVP48_RS00290	49530..49796	+	267	Protein_57	IS66 family insertion sequence element accessory protein TnpB	-
FVP48_RS00295	49816..51386	+	1571	Protein_58	IS66 family transposase	-
FVP48_RS00300	51426..51701	+	276	WP_001707307.1	hypothetical protein	-
-	51677..51838	+	162	NuclAT_0	-	-
-	51677..51838	+	162	NuclAT_0	-	-
-	51677..51838	+	162	NuclAT_0	-	-
-	51677..51838	+	162	NuclAT_0	-	-
-	51771..51836	+	66	-	-	Antitoxin
FVP48_RS00305	51882..52040	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
FVP48_RS00310	52518..52712	+	195	WP_173002228.1	hypothetical protein	-
FVP48_RS00315	52939..53226	+	288	WP_173002171.1	hypothetical protein	-
FVP48_RS00320	53345..54166	+	822	WP_173002229.1	DUF945 domain-containing protein	-
FVP48_RS00325	54463..55020	-	558	Protein_64	transglycosylase SLT domain-containing protein	-
FVP48_RS00330	55396..55779	+	384	WP_173002172.1	relaxosome protein TraM	-
FVP48_RS00335	55973..56664	+	692	Protein_66	PAS domain-containing protein	-
FVP48_RS00340	56752..56979	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	aap/aspU	1..167966	167966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T136420 WP_001302184.1 NZ_CP042885:51882-52040 [Escherichia coli O10:H32]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T136420 NZ_CP042885:51882-52040 [Escherichia coli O10:H32]
ATGAAACTACCACGCAGCTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT136420 NZ_CP042885:51771-51836 [Escherichia coli O10:H32]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin