Detailed information of TA system TA136415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 150101..150846
• Replicon: plasmid pNMBU-W07E18_01
• Accession: NZ_CP042883
• Organism: Klebsiella pneumoniae strain NMBU-W07E18

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: FVP31_RS26600
• Protein ID: WP_032408901.1
• Coordinates: 150355..150846 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: FVP31_RS26595
• Protein ID: WP_014386183.1
• Coordinates: 150101..150367 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVP31_RS26545	145653..146066	-	414	WP_013023817.1	helix-turn-helix domain-containing protein	-
FVP31_RS26550	146067..146345	-	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
FVP31_RS26555	146335..146655	-	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FVP31_RS26560	146736..146960	-	225	WP_014386189.1	hypothetical protein	-
FVP31_RS26565	146971..147183	-	213	WP_014386188.1	hypothetical protein	-
FVP31_RS26570	147245..147571	-	327	WP_014386187.1	hypothetical protein	-
FVP31_RS26575	148208..148558	-	351	WP_014386186.1	hypothetical protein	-
FVP31_RS26580	148555..148827	-	273	WP_032408902.1	hypothetical protein	-
FVP31_RS26585	149017..149502	+	486	WP_014386185.1	hypothetical protein	-
FVP31_RS26590	149746..149904	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
FVP31_RS26595	150101..150367	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
FVP31_RS26600	150355..150846	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
FVP31_RS26605	151287..151538	-	252	WP_032408900.1	aldo/keto reductase	-
FVP31_RS26610	151735..153327	-	1593	Protein_163	IS66 family transposase	-
FVP31_RS26615	153358..153708	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
FVP31_RS26620	153705..154145	-	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
FVP31_RS26625	154407..155162	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / blaTEM-1B	-	1..194856	194856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T136415 WP_032408901.1 NZ_CP042883:150355-150846 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T136415 NZ_CP042883:150355-150846 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT136415 WP_014386183.1 NZ_CP042883:150101-150367 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT136415 NZ_CP042883:150101-150367 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6