Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3456500..3457118 Replicon chromosome
Accession NZ_CP042865
Organism Escherichia coli strain ATCC BAA-196

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BAA196NC_RS17000 Protein ID WP_001291435.1
Coordinates 3456900..3457118 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BAA196NC_RS16995 Protein ID WP_000344800.1
Coordinates 3456500..3456874 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAA196NC_RS16985 3451589..3452782 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BAA196NC_RS16990 3452805..3455954 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
BAA196NC_RS16995 3456500..3456874 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BAA196NC_RS17000 3456900..3457118 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BAA196NC_RS17005 3457290..3457841 + 552 WP_000102564.1 maltose O-acetyltransferase -
BAA196NC_RS17010 3457957..3458427 + 471 WP_000136192.1 YlaC family protein -
BAA196NC_RS17015 3458591..3460141 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BAA196NC_RS17020 3460183..3460536 - 354 WP_000878140.1 DUF1428 domain-containing protein -
BAA196NC_RS17030 3460915..3461226 + 312 WP_000409911.1 MGMT family protein -
BAA196NC_RS17035 3461257..3461829 - 573 WP_000779842.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T136310 WP_001291435.1 NZ_CP042865:3456900-3457118 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T136310 NZ_CP042865:3456900-3457118 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT136310 WP_000344800.1 NZ_CP042865:3456500-3456874 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT136310 NZ_CP055451:c4699824-4699582 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References