Detailed information of TA system TA136100

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2785825..2786656
• Replicon: chromosome
• Accession: NZ_CP042843
• Organism: Escherichia coli strain JME67

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: FVE23_RS13725
• Protein ID: WP_000854814.1
• Coordinates: 2786282..2786656 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: FVE23_RS13720
• Protein ID: WP_001285584.1
• Coordinates: 2785825..2786193 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE23_RS13705	2783492..2785024	+	1533	WP_001350525.1	hypothetical protein	-
FVE23_RS13710	2785021..2785467	+	447	WP_000187523.1	RadC family protein	-
FVE23_RS13715	2785530..2785751	+	222	WP_000692323.1	DUF987 domain-containing protein	-
FVE23_RS13720	2785825..2786193	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FVE23_RS13725	2786282..2786656	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FVE23_RS13730	2786653..2786847	+	195	WP_000988600.1	hypothetical protein	-
FVE23_RS22620	2786860..2786973	+	114	WP_001161660.1	DUF957 domain-containing protein	-
FVE23_RS13740	2787262..2787390	-	129	Protein_2638	transposase domain-containing protein	-
FVE23_RS13745	2787462..2787644	+	183	WP_001016348.1	hypothetical protein	-
FVE23_RS13750	2787745..2788074	-	330	WP_000450409.1	DUF496 family protein	-
FVE23_RS13755	2788246..2789304	-	1059	WP_001200891.1	FUSC family protein	-
FVE23_RS13760	2789502..2789975	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
FVE23_RS13765	2790094..2791260	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T136100 WP_000854814.1 NZ_CP042843:2786282-2786656 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T136100 NZ_CP042843:2786282-2786656 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT136100 WP_001285584.1 NZ_CP042843:2785825-2786193 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT136100 NZ_CP042843:2785825-2786193 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6