Detailed information of TA system TA135648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 2214079..2214680
• Replicon: chromosome
• Accession: NZ_CP042392
• Organism: Loigolactobacillus coryniformis strain CBA3616

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: FGL77_RS10610
• Protein ID: WP_146990031.1
• Coordinates: 2214079..2214423 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: FGL77_RS10615
• Protein ID: WP_146990033.1
• Coordinates: 2214417..2214680 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGL77_RS10600	2211081..2212733	+	1653	WP_146990025.1	type I restriction-modification system subunit M	-
FGL77_RS10605	2212744..2213970	+	1227	WP_146990028.1	restriction endonuclease subunit S	-
FGL77_RS10610	2214079..2214423	-	345	WP_146990031.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FGL77_RS10615	2214417..2214680	-	264	WP_146990033.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FGL77_RS10620	2214766..2215353	+	588	WP_146990036.1	site-specific integrase	-
FGL77_RS10625	2215451..2216716	+	1266	WP_146990038.1	topoisomerase IV	-
FGL77_RS10635	2217299..2218057	-	759	WP_003680852.1	IS21-like element helper ATPase IstB	-
FGL77_RS10640	2218044..2219606	-	1563	WP_010013312.1	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13039.85 Da
• Isoelectric Point: 6.4782

>T135648 WP_146990031.1 NZ_CP042392:c2214423-2214079 [Loigolactobacillus coryniformis]
MVSQGDIFYVNFNPSRGHEQINKRPAISLSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T135648 NZ_CP042392:c2214423-2214079 [Loigolactobacillus coryniformis]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATAAATAAGCGACCAGCCAT
TTCTCTAAGCAATGATTTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10175.48 Da
• Isoelectric Point: 5.7117

>AT135648 WP_146990033.1 NZ_CP042392:c2214680-2214417 [Loigolactobacillus coryniformis]
MEFIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT135648 NZ_CP042392:c2214680-2214417 [Loigolactobacillus coryniformis]
ATGGAATTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA