Detailed information of TA system TA135511

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4847370..4848168
• Replicon: chromosome
• Accession: NZ_CP042350
• Organism: Escherichia coli strain RM10410

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: FS611_RS24225
• Protein ID: WP_040076706.1
• Coordinates: 4847370..4847747 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FS611_RS24230
• Protein ID: WP_040076708.1
• Coordinates: 4847794..4848168 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS611_RS24195	4842647..4844185	-	1539	WP_001187173.1	lysine decarboxylation/transport transcriptional activator CadC	-
FS611_RS24200	4845067..4845393	-	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FS611_RS24205	4845390..4845653	-	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
FS611_RS24210	4845725..4846591	-	867	WP_000014510.1	DUF4942 domain-containing protein	-
FS611_RS24215	4846676..4846873	-	198	WP_000839256.1	DUF957 domain-containing protein	-
FS611_RS24220	4846885..4847373	-	489	WP_000761662.1	hypothetical protein	-
FS611_RS24225	4847370..4847747	-	378	WP_040076706.1	TA system toxin CbtA family protein	Toxin
FS611_RS24230	4847794..4848168	-	375	WP_040076708.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FS611_RS24235	4848331..4848552	-	222	WP_000692358.1	DUF987 domain-containing protein	-
FS611_RS24240	4848615..4849091	-	477	WP_001186727.1	RadC family protein	-
FS611_RS24245	4849107..4849592	-	486	WP_000214386.1	antirestriction protein	-
FS611_RS24250	4849682..4850500	-	819	WP_033884686.1	DUF945 domain-containing protein	-
FS611_RS24255	4850591..4850824	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4835159..4860863	25704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14180.10 Da
• Isoelectric Point: 7.2920

>T135511 WP_040076706.1 NZ_CP042350:c4847747-4847370 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T135511 NZ_CP042350:c4847747-4847370 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13694.44 Da
• Isoelectric Point: 6.4757

>AT135511 WP_040076708.1 NZ_CP042350:c4848168-4847794 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVKPCFGARLVQEGNRLHYLADRAGISSRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHSVTLYAKGLTCEADTLGSCGYVYLTVYPTPATATTS

Nucleotide

• Length: 375 bp

>AT135511 NZ_CP042350:c4848168-4847794 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAA
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGCAGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATTCCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCGGCAACCGCAACCACCTCATAA