Detailed information of TA system TA135506

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3591712..3592549
• Replicon: chromosome
• Accession: NZ_CP042350
• Organism: Escherichia coli strain RM10410

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: FS611_RS18155
• Protein ID: WP_000854687.1
• Coordinates: 3591712..3592092 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FS611_RS18160
• Protein ID: WP_012816826.1
• Coordinates: 3592181..3592549 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS611_RS18125	3587354..3589195	+	1842	WP_000437375.1	RNA polymerase sigma factor RpoD	-
FS611_RS18130	3589274..3589780	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
FS611_RS18140	3590080..3590925	-	846	WP_001274558.1	DUF4942 domain-containing protein	-
FS611_RS18145	3591010..3591207	-	198	WP_040077060.1	DUF957 domain-containing protein	-
FS611_RS18150	3591227..3591715	-	489	WP_001054227.1	hypothetical protein	-
FS611_RS18155	3591712..3592092	-	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
FS611_RS18160	3592181..3592549	-	369	WP_012816826.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FS611_RS18165	3592624..3592845	-	222	WP_000692340.1	DUF987 domain-containing protein	-
FS611_RS18170	3592914..3593390	-	477	WP_001364966.1	RadC family protein	-
FS611_RS18175	3593406..3593879	-	474	WP_040076670.1	antirestriction protein	-
FS611_RS18180	3593973..3594218	-	246	WP_000680588.1	hypothetical protein	-
FS611_RS18185	3594218..3595039	-	822	WP_148050532.1	DUF945 domain-containing protein	-
FS611_RS18195	3595536..3596744	+	1209	WP_040077378.1	IS4-like element ISVsa5 family transposase	-
FS611_RS18200	3596759..3597007	-	249	Protein_3464	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3595536..3596744	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T135506 WP_000854687.1 NZ_CP042350:c3592092-3591712 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T135506 NZ_CP042350:c3592092-3591712 [Escherichia coli]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13780.56 Da
• Isoelectric Point: 6.4763

>AT135506 WP_012816826.1 NZ_CP042350:c3592549-3592181 [Escherichia coli]
VSDTLHETNYPNDNHNRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT135506 NZ_CP042350:c3592549-3592181 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACAACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67