Detailed information of TA system TA135416

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1211117..1211952
• Replicon: chromosome
• Accession: NZ_CP042336
• Organism: Escherichia coli strain GZ04-0086

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: E3B71_RS08115
• Protein ID: WP_000854761.1
• Coordinates: 1211575..1211952 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: E3B71_RS08110
• Protein ID: WP_001295723.1
• Coordinates: 1211117..1211485 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3B71_RS08075	1208232..1208390	+	159	WP_001323397.1	DUF905 family protein	-
E3B71_RS08085	1208545..1209363	+	819	WP_046464190.1	DUF945 domain-containing protein	-
E3B71_RS08095	1209705..1210178	+	474	WP_000855059.1	antirestriction protein	-
E3B71_RS08100	1210194..1210670	+	477	WP_001186774.1	RadC family protein	-
E3B71_RS08105	1210733..1210954	+	222	WP_000692345.1	DUF987 domain-containing protein	-
E3B71_RS08110	1211117..1211485	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E3B71_RS08115	1211575..1211952	+	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
E3B71_RS08120	1211949..1212151	+	203	Protein_1208	hypothetical protein	-
E3B71_RS08125	1212164..1212277	+	114	WP_001161660.1	DUF957 domain-containing protein	-
E3B71_RS08130	1212712..1214247	-	1536	WP_001333339.1	IS66-like element ISEc22 family transposase	-
E3B71_RS08135	1214296..1214643	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
E3B71_RS08140	1214640..1215044	-	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
E3B71_RS08145	1215556..1215927	+	372	WP_001295631.1	IS110 family transposase	-
E3B71_RS08155	1216381..1216563	+	183	WP_001016348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T135416 WP_000854761.1 NZ_CP042336:1211575-1211952 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T135416 NZ_CP042336:1211575-1211952 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT135416 WP_001295723.1 NZ_CP042336:1211117-1211485 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT135416 NZ_CP042336:1211117-1211485 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52