Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3994220..3995052 Replicon chromosome
Accession NZ_CP042298
Organism Escherichia coli strain RM9088

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag FRV13_RS21775 Protein ID WP_000854753.1
Coordinates 3994220..3994594 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID E3PJ72
Locus tag FRV13_RS21780 Protein ID WP_001278232.1
Coordinates 3994684..3995052 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FRV13_RS21735 3989336..3989872 + 537 WP_001411126.1 GspM family type II secretion system protein YghD -
FRV13_RS21740 3990153..3990611 - 459 Protein_3898 DUF4942 domain-containing protein -
FRV13_RS21750 3990665..3991342 + 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -
FRV13_RS21755 3991342..3991689 + 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
FRV13_RS21760 3991709..3993280 + 1572 WP_000381395.1 IS66 family transposase -
FRV13_RS28375 3993355..3993429 - 75 Protein_3902 restriction endonuclease subunit M -
FRV13_RS21765 3993526..3993723 - 198 WP_000839281.1 DUF957 domain-containing protein -
FRV13_RS21770 3993735..3994223 - 489 WP_000777541.1 hypothetical protein -
FRV13_RS21775 3994220..3994594 - 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
FRV13_RS21780 3994684..3995052 - 369 WP_001278232.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
FRV13_RS21785 3995215..3995436 - 222 WP_000692316.1 DUF987 domain-containing protein -
FRV13_RS21790 3995499..3995975 - 477 WP_001186726.1 RadC family protein -
FRV13_RS21795 3995991..3996476 - 486 WP_047084650.1 antirestriction protein -
FRV13_RS21800 3996531..3996773 - 243 Protein_3910 DUF945 domain-containing protein -
FRV13_RS21805 3996834..3998047 + 1214 WP_169302185.1 IS3 family transposase -
FRV13_RS21810 3998090..3998662 - 573 Protein_3912 DUF945 domain-containing protein -
FRV13_RS21820 3998762..3998995 - 234 WP_001119729.1 DUF905 family protein -
FRV13_RS21825 3999074..3999529 - 456 WP_000581504.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 3990695..3998047 7352


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T135187 WP_000854753.1 NZ_CP042298:c3994594-3994220 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T135187 NZ_CP042298:c3994594-3994220 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13491.37 Da        Isoelectric Point: 6.6290

>AT135187 WP_001278232.1 NZ_CP042298:c3995052-3994684 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT135187 NZ_CP042298:c3995052-3994684 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB E3PJ72

References