Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 555797..556415 Replicon chromosome
Accession NZ_CP042298
Organism Escherichia coli strain RM9088

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FRV13_RS04205 Protein ID WP_001291435.1
Coordinates 555797..556015 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FRV13_RS04210 Protein ID WP_000344800.1
Coordinates 556041..556415 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FRV13_RS04170 551087..551659 + 573 WP_047084960.1 YbaY family lipoprotein -
FRV13_RS04175 551690..552001 - 312 WP_000409911.1 MGMT family protein -
FRV13_RS04185 552380..552733 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FRV13_RS04190 552775..554325 - 1551 WP_001514985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FRV13_RS04195 554489..554959 - 471 WP_000136192.1 YlaC family protein -
FRV13_RS04200 555075..555626 - 552 WP_000102564.1 maltose O-acetyltransferase -
FRV13_RS04205 555797..556015 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FRV13_RS04210 556041..556415 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FRV13_RS04215 556961..560110 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FRV13_RS04220 560133..561326 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T135168 WP_001291435.1 NZ_CP042298:c556015-555797 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T135168 NZ_CP042298:c556015-555797 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT135168 WP_000344800.1 NZ_CP042298:c556415-556041 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT135168 NZ_CP042298:c556415-556041 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References