Detailed information of TA system TA135157

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 134154..134407
• Replicon: plasmid p1RM9088
• Accession: NZ_CP042296
• Organism: Escherichia coli strain RM9088

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: FRV13_RS00790
• Protein ID: WP_001312851.1
• Coordinates: 134154..134303 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	134351..134407 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRV13_RS00760	129669..130133	-	465	WP_040090730.1	hypothetical protein	-
FRV13_RS00765	130412..131452	-	1041	WP_139320987.1	hypothetical protein	-
FRV13_RS28155	131562..131750	-	189	WP_169301556.1	hypothetical protein	-
FRV13_RS00770	132454..133311	-	858	WP_000829162.1	incFII family plasmid replication initiator RepA	-
FRV13_RS00775	133304..133378	-	75	WP_001364037.1	RepA leader peptide Tap	-
FRV13_RS00785	133622..133870	-	249	WP_000083838.1	replication regulatory protein RepA	-
FRV13_RS00790	134154..134303	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	134351..134407	+	57	NuclAT_0	-	Antitoxin
-	134351..134407	+	57	NuclAT_0	-	Antitoxin
-	134351..134407	+	57	NuclAT_0	-	Antitoxin
-	134351..134407	+	57	NuclAT_0	-	Antitoxin
FRV13_RS00800	134559..135023	-	465	WP_042966773.1	hypothetical protein	-
FRV13_RS00805	135178..135768	-	591	WP_000766788.1	DUF2726 domain-containing protein	-
FRV13_RS00810	135806..136015	-	210	WP_001355319.1	hemolysin expression modulator Hha	-
FRV13_RS00820	136343..136555	-	213	WP_001364091.1	hypothetical protein	-
FRV13_RS00825	136726..137403	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
FRV13_RS00830	137403..137750	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
FRV13_RS00835	137770..139341	+	1572	WP_000381395.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyD / hlyB / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..167256	167256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T135157 WP_001312851.1 NZ_CP042296:c134303-134154 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T135157 NZ_CP042296:c134303-134154 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCTGTGTGCGCTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT135157 NZ_CP042296:134351-134407 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATCTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin