Detailed information of TA system TA135142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3332779..3333416
• Replicon: chromosome
• Accession: NZ_CP042271
• Organism: Bacillus velezensis strain LPL061

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: FQV73_RS16350
• Protein ID: WP_003156187.1
• Coordinates: 3333066..3333416 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: FQV73_RS16345
• Protein ID: WP_003156188.1
• Coordinates: 3332779..3333060 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQV73_RS16325	3329144..3329743	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
FQV73_RS16330	3329836..3330201	+	366	WP_014304402.1	holo-ACP synthase	-
FQV73_RS16335	3330366..3331373	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
FQV73_RS16340	3331490..3332659	+	1170	WP_003156189.1	alanine racemase	-
FQV73_RS16345	3332779..3333060	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
FQV73_RS16350	3333066..3333416	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FQV73_RS16355	3333534..3334355	+	822	WP_003156182.1	STAS domain-containing protein	-
FQV73_RS16360	3334360..3334725	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
FQV73_RS16365	3334728..3335129	+	402	WP_003156178.1	anti-sigma regulatory factor	-
FQV73_RS16370	3335141..3336148	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
FQV73_RS16375	3336212..3336541	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
FQV73_RS16380	3336538..3337020	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
FQV73_RS16385	3336986..3337774	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
FQV73_RS16390	3337774..3338376	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T135142 WP_003156187.1 NZ_CP042271:3333066-3333416 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T135142 NZ_CP042271:3333066-3333416 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT135142 WP_003156188.1 NZ_CP042271:3332779-3333060 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT135142 NZ_CP042271:3332779-3333060 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5