Detailed information of TA system TA135138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5097000..5097595
• Replicon: chromosome
• Accession: NZ_CP042269
• Organism: Pseudomonas aeruginosa strain HOU1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: FQ758_RS24990
• Protein ID: WP_003113526.1
• Coordinates: 5097317..5097595 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FQ758_RS24985
• Protein ID: WP_003099268.1
• Coordinates: 5097000..5097305 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQ758_RS24950	5092140..5092988	+	849	WP_003117426.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
FQ758_RS24960	5093155..5094096	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
FQ758_RS24965	5094213..5094827	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
FQ758_RS24970	5094869..5095453	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
FQ758_RS24975	5095494..5096594	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
FQ758_RS24985	5097000..5097305	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
FQ758_RS24990	5097317..5097595	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FQ758_RS25000	5097923..5100151	+	2229	WP_003099265.1	TonB-dependent receptor	-
FQ758_RS25005	5100221..5100868	-	648	WP_003095021.1	carbonate dehydratase	-
FQ758_RS25010	5100930..5102168	-	1239	WP_116818957.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T135138 WP_003113526.1 NZ_CP042269:c5097595-5097317 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T135138 NZ_CP042269:c5097595-5097317 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGTTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT135138 WP_003099268.1 NZ_CP042269:c5097305-5097000 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT135138 NZ_CP042269:c5097305-5097000 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3