Detailed information of TA system TA135073

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 107408..107834
• Replicon: plasmid pBCE049-1
• Accession: NZ_CP042247
• Organism: Escherichia coli strain BCE049

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FQU84_RS00585
• Protein ID: WP_001312861.1
• Coordinates: 107408..107566 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	107610..107834 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU84_RS00545	102471..102698	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
FQU84_RS00550	102792..103478	-	687	WP_000332492.1	PAS domain-containing protein	-
FQU84_RS00555	103668..104051	-	384	WP_000124981.1	relaxosome protein TraM	-
FQU84_RS00565	104374..104976	+	603	WP_000243707.1	transglycosylase SLT domain-containing protein	-
FQU84_RS00570	105272..106093	-	822	Protein_101	DUF945 domain-containing protein	-
FQU84_RS00575	106213..106500	-	288	WP_000107542.1	hypothetical protein	-
FQU84_RS00580	106525..106731	-	207	WP_032192990.1	hypothetical protein	-
FQU84_RS00585	107408..107566	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	107610..107834	-	225	NuclAT_0	-	Antitoxin
-	107610..107834	-	225	NuclAT_0	-	Antitoxin
-	107610..107834	-	225	NuclAT_0	-	Antitoxin
-	107610..107834	-	225	NuclAT_0	-	Antitoxin
FQU84_RS27035	107646..107834	+	189	WP_001299721.1	hypothetical protein	-
FQU84_RS00590	107846..108565	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
FQU84_RS00595	108562..108996	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FQU84_RS00600	109051..111009	-	1959	WP_097501884.1	ParB/RepB/Spo0J family partition protein	-
FQU84_RS00605	111075..111308	-	234	WP_000005990.1	DUF905 family protein	-
FQU84_RS00610	111371..111868	-	498	WP_085457280.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cdtC / cdtB / cdtA / cnf1 / hlyD / hlyB / hlyA / hlyC / iutA / iucD / iucC / iucB / iucA	1..142705	142705

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T135073 WP_001312861.1 NZ_CP042247:c107566-107408 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T135073 NZ_CP042247:c107566-107408 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT135073 NZ_CP042247:c107834-107610 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin