Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2254559..2255185 Replicon chromosome
Accession NZ_CP041866
Organism Mycobacterium tuberculosis strain 1-0036P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag FPJ40_RS10430 Protein ID WP_003410075.1
Coordinates 2254787..2255185 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag FPJ40_RS10425 Protein ID WP_003911750.1
Coordinates 2254559..2254786 (+) Length 76 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ40_RS10415 2252598..2252942 - 345 WP_003410065.1 ferredoxin family protein -
FPJ40_RS10420 2253131..2254300 - 1170 WP_003899126.1 ATP-binding protein -
FPJ40_RS10425 2254559..2254786 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FPJ40_RS10430 2254787..2255185 + 399 WP_003410075.1 PIN domain nuclease Toxin
FPJ40_RS10435 2255368..2255799 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
FPJ40_RS10440 2255900..2256334 + 435 WP_003914358.1 DUF1398 domain-containing protein -
FPJ40_RS10445 2256771..2256950 - 180 Protein_2064 hypothetical protein -
FPJ40_RS10450 2257011..2257658 + 648 WP_071854215.1 transposase -
FPJ40_RS10455 2257612..2258202 + 591 WP_003899131.1 IS110 family transposase -
FPJ40_RS10460 2258330..2259586 - 1257 WP_003410095.1 HNH endonuclease -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-120)

Antitoxin

(2-43)


Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T133991 WP_003410075.1 NZ_CP041866:2254787-2255185 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T133991 NZ_CP041866:2254787-2255185 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT133991 WP_003911750.1 NZ_CP041866:2254559-2254786 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT133991 NZ_CP041866:2254559-2254786 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4CHG


Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References