Detailed information of TA system TA133761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2886053..2886684
• Replicon: chromosome
• Accession: NZ_CP041862
• Organism: Mycobacterium tuberculosis strain 1-0047P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPJ44_RS13410
• Protein ID: WP_003413174.1
• Coordinates: 2886307..2886684 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ44_RS13405
• Protein ID: WP_003413167.1
• Coordinates: 2886053..2886310 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ44_RS13375	2881967..2882281	+	315	WP_009937839.1	hypothetical protein	-
FPJ44_RS13380	2882577..2883788	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ44_RS13385	2883915..2884574	+	660	WP_003900846.1	LppA family lipoprotein	-
FPJ44_RS13390	2884571..2885233	+	663	WP_023643170.1	hypothetical protein	-
FPJ44_RS13395	2885230..2885508	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ44_RS13400	2885601..2886014	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ44_RS13405	2886053..2886310	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ44_RS13410	2886307..2886684	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ44_RS13415	2886700..2887074	-	375	WP_003413177.1	hypothetical protein	-
FPJ44_RS13420	2887174..2887569	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ44_RS13425	2887566..2887811	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ44_RS13430	2888222..2888641	-	420	WP_003413190.1	A24 family peptidase	-
FPJ44_RS13435	2888653..2889462	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ44_RS13440	2889459..2890712	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ44_RS13445	2890705..2891217	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T133761 WP_003413174.1 NZ_CP041862:2886307-2886684 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T133761 NZ_CP041862:2886307-2886684 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT133761 WP_003413167.1 NZ_CP041862:2886053-2886310 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT133761 NZ_CP041862:2886053-2886310 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5