Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3138601..3139171 Replicon chromosome
Accession NZ_CP041853
Organism Mycobacterium tuberculosis strain 1-0107P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FPJ55_RS14790 Protein ID WP_003414166.1
Coordinates 3138601..3138957 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FPJ55_RS14795 Protein ID WP_003901465.1
Coordinates 3138941..3139171 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ55_RS14770 3134053..3135741 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FPJ55_RS14775 3135745..3136071 - 327 WP_003414157.1 hypothetical protein -
FPJ55_RS14780 3136244..3136831 + 588 WP_003914429.1 DUF3558 family protein -
FPJ55_RS14785 3136850..3138499 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FPJ55_RS14790 3138601..3138957 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FPJ55_RS14795 3138941..3139171 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FPJ55_RS14800 3139214..3140257 - 1044 WP_016720604.1 DUF2293 domain-containing protein -
FPJ55_RS14805 3140448..3140723 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FPJ55_RS14810 3140899..3141153 - 255 WP_155936531.1 hypothetical protein -
FPJ55_RS14815 3141301..3141693 + 393 WP_078397589.1 hypothetical protein -
FPJ55_RS14820 3141690..3141881 + 192 WP_003414184.1 hypothetical protein -
FPJ55_RS14825 3141945..3143233 + 1289 Protein_2926 transposase family protein -
FPJ55_RS14830 3143467..3143694 + 228 Protein_2927 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3132250..3165779 33529


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T133228 WP_003414166.1 NZ_CP041853:c3138957-3138601 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T133228 NZ_CP041853:c3138957-3138601 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT133228 WP_003901465.1 NZ_CP041853:c3139171-3138941 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT133228 NZ_CP041853:c3139171-3138941 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References