Detailed information of TA system TA133227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3099017..3099704
• Replicon: chromosome
• Accession: NZ_CP041853
• Organism: Mycobacterium tuberculosis strain 1-0107P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: FPJ55_RS14580
• Protein ID: WP_003414064.1
• Coordinates: 3099309..3099704 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPJ55_RS14575
• Protein ID: WP_003414061.1
• Coordinates: 3099017..3099283 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ55_RS14550	3094656..3095558	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FPJ55_RS14555	3095627..3096379	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPJ55_RS14560	3096623..3096898	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPJ55_RS14565	3096895..3098517	-	1623	WP_015630537.1	type I restriction-modification system subunit M	-
FPJ55_RS14570	3098604..3099020	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
FPJ55_RS14575	3099017..3099283	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ55_RS14580	3099309..3099704	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ55_RS14585	3099701..3099970	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ55_RS14590	3099980..3101074	-	1095	WP_015630539.1	restriction endonuclease subunit S	-
FPJ55_RS14595	3101071..3101490	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPJ55_RS14600	3101564..3102043	-	480	WP_003414073.1	dihydrofolate reductase	-
FPJ55_RS14605	3102114..3102914	-	801	WP_003911953.1	thymidylate synthase	-
FPJ55_RS14610	3103070..3103807	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T133227 WP_003414064.1 NZ_CP041853:c3099704-3099309 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T133227 NZ_CP041853:c3099704-3099309 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT133227 WP_003414061.1 NZ_CP041853:c3099283-3099017 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT133227 NZ_CP041853:c3099283-3099017 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64