Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2884862..2885432 Replicon chromosome
Accession NZ_CP041836
Organism Mycobacterium tuberculosis strain 2-0031P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FPJ72_RS13480 Protein ID WP_003414166.1
Coordinates 2884862..2885218 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FPJ72_RS13485 Protein ID WP_003901465.1
Coordinates 2885202..2885432 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ72_RS13460 2880314..2882002 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FPJ72_RS13465 2882006..2882332 - 327 WP_003414157.1 hypothetical protein -
FPJ72_RS13470 2882505..2883092 + 588 WP_003914429.1 DUF3558 family protein -
FPJ72_RS13475 2883111..2884760 + 1650 Protein_2665 CocE/NonD family hydrolase -
FPJ72_RS13480 2884862..2885218 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FPJ72_RS13485 2885202..2885432 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FPJ72_RS13490 2885475..2886518 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
FPJ72_RS13495 2886709..2886984 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FPJ72_RS13500 2887160..2887414 - 255 WP_003917684.1 hypothetical protein -
FPJ72_RS13505 2887562..2887966 + 405 WP_003414181.1 hypothetical protein -
FPJ72_RS13510 2887963..2888154 + 192 WP_003414184.1 hypothetical protein -
FPJ72_RS13515 2888218..2889507 + 1290 Protein_2673 transposase family protein -
FPJ72_RS13520 2889741..2889998 + 258 WP_003899489.1 hypothetical protein -
FPJ72_RS13525 2890103..2890414 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T132193 WP_003414166.1 NZ_CP041836:c2885218-2884862 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T132193 NZ_CP041836:c2885218-2884862 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT132193 WP_003901465.1 NZ_CP041836:c2885432-2885202 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT132193 NZ_CP041836:c2885432-2885202 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References