Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3541632..3542302 Replicon chromosome
Accession NZ_CP041832
Organism Mycobacterium tuberculosis strain 2-0052P6C4

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FPJ76_RS16620 Protein ID WP_003899954.1
Coordinates 3541632..3541976 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FPJ76_RS16625 Protein ID WP_003899955.1
Coordinates 3541973..3542302 (+) Length 110 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ76_RS16590 3536705..3537565 + 861 WP_003416628.1 alpha/beta hydrolase -
FPJ76_RS16595 3537540..3538055 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FPJ76_RS16600 3538295..3538588 + 294 WP_003416635.1 hypothetical protein -
FPJ76_RS16605 3538876..3540165 + 1290 WP_003416640.1 ATP-binding protein -
FPJ76_RS16610 3540512..3540946 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FPJ76_RS16615 3540949..3541401 - 453 WP_193065618.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPJ76_RS16620 3541632..3541976 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPJ76_RS16625 3541973..3542302 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FPJ76_RS16630 3542840..3543187 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FPJ76_RS16635 3543184..3543804 + 621 WP_031682106.1 RES family NAD+ phosphorylase -
FPJ76_RS16640 3543964..3545229 - 1266 WP_003902423.1 hypothetical protein -
FPJ76_RS16645 3545397..3545606 + 210 WP_003416778.1 hypothetical protein -
FPJ76_RS16650 3545853..3546887 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-103)

Antitoxin

(38-91)


Sequences


Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T131966 WP_003899954.1 NZ_CP041832:3541632-3541976 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T131966 NZ_CP041832:3541632-3541976 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT131966 WP_003899955.1 NZ_CP041832:3541973-3542302 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT131966 NZ_CP041832:3541973-3542302 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2


Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References