Detailed information of TA system TA131955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2859062..2859693
• Replicon: chromosome
• Accession: NZ_CP041832
• Organism: Mycobacterium tuberculosis strain 2-0052P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPJ76_RS13300
• Protein ID: WP_031682061.1
• Coordinates: 2859316..2859693 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ76_RS13295
• Protein ID: WP_003413167.1
• Coordinates: 2859062..2859319 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ76_RS13265	2854977..2855291	+	315	WP_009937839.1	hypothetical protein	-
FPJ76_RS13270	2855587..2856798	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ76_RS13275	2856925..2857584	+	660	WP_003902296.1	hypothetical protein	-
FPJ76_RS13280	2857581..2858243	+	663	WP_003905878.1	hypothetical protein	-
FPJ76_RS13285	2858240..2858517	+	278	Protein_2622	type II toxin-antitoxin system VapB family antitoxin	-
FPJ76_RS13290	2858610..2859023	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ76_RS13295	2859062..2859319	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ76_RS13300	2859316..2859693	+	378	WP_031682061.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ76_RS13305	2859709..2860083	-	375	WP_003413177.1	hypothetical protein	-
FPJ76_RS13310	2860183..2860578	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ76_RS13315	2860575..2860820	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ76_RS13320	2861231..2861650	-	420	WP_003413190.1	A24 family peptidase	-
FPJ76_RS13325	2861662..2862471	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ76_RS13330	2862468..2863721	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ76_RS13335	2863714..2864226	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13758.74 Da
• Isoelectric Point: 4.4687

>T131955 WP_031682061.1 NZ_CP041832:2859316-2859693 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELTALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T131955 NZ_CP041832:2859316-2859693 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCACGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT131955 WP_003413167.1 NZ_CP041832:2859062-2859319 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT131955 NZ_CP041832:2859062-2859319 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829C9W5