Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2867877..2868514 Replicon chromosome
Accession NZ_CP041824
Organism Mycobacterium tuberculosis strain 4-0012P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag FPJ84_RS13300 Protein ID WP_003413180.1
Coordinates 2867877..2868272 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag FPJ84_RS13305 Protein ID WP_003413183.1
Coordinates 2868269..2868514 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ84_RS13260 2863962..2864621 + 660 WP_003900846.1 LppA family lipoprotein -
FPJ84_RS13265 2864618..2865277 + 660 WP_003900847.1 hypothetical protein -
FPJ84_RS13270 2865274..2865936 + 663 WP_003900848.1 hypothetical protein -
FPJ84_RS13275 2865933..2866211 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
FPJ84_RS13280 2866304..2866717 + 414 WP_003413164.1 PIN domain nuclease -
FPJ84_RS13285 2866756..2867013 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
FPJ84_RS13290 2867010..2867387 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
FPJ84_RS13295 2867403..2867777 - 375 WP_003413177.1 hypothetical protein -
FPJ84_RS13300 2867877..2868272 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
FPJ84_RS13305 2868269..2868514 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
FPJ84_RS13310 2868925..2869344 - 420 WP_003413190.1 A24 family peptidase -
FPJ84_RS13315 2869356..2870165 - 810 WP_003413193.1 shikimate dehydrogenase -
FPJ84_RS13320 2870162..2871418 - 1257 WP_014585306.1 endolytic transglycosylase MltG -
FPJ84_RS13325 2871411..2871923 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T131474 WP_003413180.1 NZ_CP041824:c2868272-2867877 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T131474 NZ_CP041824:c2868272-2867877 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT131474 WP_003413183.1 NZ_CP041824:c2868514-2868269 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT131474 NZ_CP041824:c2868514-2868269 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References