Detailed information of TA system TA130915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2643077..2643708
• Replicon: chromosome
• Accession: NZ_CP041815
• Organism: Mycobacterium tuberculosis strain SEA02010036P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPJ94_RS12160
• Protein ID: WP_003413174.1
• Coordinates: 2643331..2643708 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ94_RS12155
• Protein ID: WP_003413167.1
• Coordinates: 2643077..2643334 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ94_RS12125	2638992..2639306	+	315	WP_009937839.1	hypothetical protein	-
FPJ94_RS12130	2639602..2640813	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ94_RS12135	2640940..2641599	+	660	WP_003902296.1	hypothetical protein	-
FPJ94_RS12140	2641596..2642258	+	663	WP_003905878.1	hypothetical protein	-
FPJ94_RS12145	2642255..2642532	+	278	Protein_2403	type II toxin-antitoxin system VapB family antitoxin	-
FPJ94_RS12150	2642625..2643038	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ94_RS12155	2643077..2643334	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ94_RS12160	2643331..2643708	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ94_RS12165	2643724..2644098	-	375	WP_003413177.1	hypothetical protein	-
FPJ94_RS12170	2644198..2644593	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ94_RS12175	2644590..2644835	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ94_RS12180	2645246..2645665	-	420	WP_003413190.1	A24 family peptidase	-
FPJ94_RS12185	2645677..2646486	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ94_RS12190	2646483..2647736	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ94_RS12195	2647729..2648241	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T130915 WP_003413174.1 NZ_CP041815:2643331-2643708 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T130915 NZ_CP041815:2643331-2643708 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT130915 WP_003413167.1 NZ_CP041815:2643077-2643334 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT130915 NZ_CP041815:2643077-2643334 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5