Detailed information of TA system TA129023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 6302769..6303436
• Replicon: chromosome
• Accession: NZ_CP041695
• Organism: Nocardia otitidiscaviarum strain NEB252

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOH10_RS28210
• Protein ID: WP_143982960.1
• Coordinates: 6302769..6303185 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FOH10_RS28215
• Protein ID: WP_143982961.1
• Coordinates: 6303182..6303436 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH10_RS28180	6297891..6298412	-	522	WP_143982956.1	hypothetical protein	-
FOH10_RS28185	6299070..6299645	-	576	WP_186000615.1	MspA family porin	-
FOH10_RS28190	6300081..6300737	+	657	WP_143982958.1	hypothetical protein	-
FOH10_RS28195	6300787..6300987	-	201	WP_143982959.1	hypothetical protein	-
FOH10_RS28200	6300984..6301196	-	213	WP_029926459.1	hypothetical protein	-
FOH10_RS35045	6301199..6301363	-	165	WP_186000616.1	hypothetical protein	-
FOH10_RS28205	6301562..6302764	+	1203	WP_143984360.1	transcriptional regulator	-
FOH10_RS28210	6302769..6303185	-	417	WP_143982960.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOH10_RS28215	6303182..6303436	-	255	WP_143982961.1	Arc family DNA-binding protein	Antitoxin
FOH10_RS28220	6303501..6304820	-	1320	WP_143982962.1	M18 family aminopeptidase	-
FOH10_RS28225	6304975..6305412	-	438	WP_143982963.1	MarR family transcriptional regulator	-
FOH10_RS28230	6305458..6306537	+	1080	WP_143982964.1	substrate-binding domain-containing protein	-
FOH10_RS28235	6306538..6307215	-	678	WP_143982965.1	phosphoribosylformylglycinamidine synthase subunit PurQ	-
FOH10_RS28240	6307212..6307445	-	234	WP_029926445.1	phosphoribosylformylglycinamidine synthase subunit PurS	-
FOH10_RS28245	6307684..6308328	+	645	WP_039809257.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (11-46)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14642.69 Da
• Isoelectric Point: 4.5388

>T129023 WP_143982960.1 NZ_CP041695:c6303185-6302769 [Nocardia otitidiscaviarum]
VIILDTNVISETSTKAPNPRVLAWLDAQNAETLFLAAVTVGELRYGIAVMPAGRRRDEYTDWLENLTIPSFSGRILPYDL
AATAQYAHLMSTARAAGLAISPPDGMIAATASAAGFAVATRDTAPFEAAGLPVINPWQ

Nucleotide

• Length: 417 bp

>T129023 NZ_CP041695:c6303185-6302769 [Nocardia otitidiscaviarum]
GTGATCATCCTGGACACCAACGTCATCAGCGAGACCTCGACCAAGGCCCCCAATCCGCGGGTACTGGCATGGCTCGACGC
CCAGAACGCCGAAACCCTCTTCCTGGCGGCGGTGACCGTGGGTGAACTCCGGTACGGCATCGCCGTCATGCCCGCCGGCC
GCCGCCGCGACGAGTACACCGATTGGCTCGAAAACCTCACCATCCCTTCGTTTTCCGGCCGCATACTGCCCTACGATCTC
GCCGCTACCGCCCAATACGCGCATCTCATGTCCACCGCCCGCGCCGCCGGGCTGGCAATCTCGCCCCCTGACGGCATGAT
CGCGGCTACCGCGTCCGCCGCAGGCTTCGCCGTCGCCACCCGCGACACCGCACCGTTCGAAGCCGCCGGATTGCCGGTCA
TCAATCCCTGGCAATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9513.71 Da
• Isoelectric Point: 5.1912

>AT129023 WP_143982961.1 NZ_CP041695:c6303436-6303182 [Nocardia otitidiscaviarum]
MKNVTIRNLSDEVHRAIRQRAATHGRSVEAEMRAILAEAVQPPERIKLGSLLAGGISEENKLTDEEHEIYFGRDRTPAEP
MEFE

Nucleotide

• Length: 255 bp

>AT129023 NZ_CP041695:c6303436-6303182 [Nocardia otitidiscaviarum]
ATGAAGAACGTGACGATCCGTAATCTCAGCGACGAGGTGCACCGCGCGATTCGCCAACGCGCGGCCACCCACGGTCGCAG
CGTCGAGGCGGAGATGCGTGCCATTCTCGCCGAGGCCGTGCAGCCACCCGAGCGGATCAAGCTCGGTTCACTACTAGCGG
GCGGCATCAGTGAAGAGAACAAACTGACCGACGAGGAACACGAAATCTACTTCGGCCGCGACCGAACCCCGGCCGAGCCC
ATGGAGTTCGAGTGA