Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4695..5338 Replicon plasmid unnamed
Accession NZ_CP041514
Organism Klebsiella michiganensis strain KNU07

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag FNA74_RS00055 Protein ID WP_020804312.1
Coordinates 4922..5338 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag FNA74_RS00050 Protein ID WP_001261276.1
Coordinates 4695..4925 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNA74_RS30560 236..349 + 114 WP_014343462.1 hypothetical protein -
FNA74_RS00005 478..735 + 258 WP_009310077.1 hypothetical protein -
FNA74_RS00010 793..1572 - 780 WP_023287113.1 site-specific integrase -
FNA74_RS00015 1770..2785 - 1016 Protein_3 hypothetical protein -
FNA74_RS00020 2819..3154 - 336 WP_009309920.1 hypothetical protein -
FNA74_RS00030 3573..3878 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB -
FNA74_RS00035 3880..4098 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
FNA74_RS00050 4695..4925 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FNA74_RS00055 4922..5338 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
FNA74_RS00060 5379..6257 - 879 WP_032427228.1 restriction endonuclease -
FNA74_RS00065 6919..8195 + 1277 Protein_10 HlyD family secretion protein -
FNA74_RS00070 8185..10293 + 2109 WP_032427226.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..203764 203764


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T128729 WP_020804312.1 NZ_CP041514:4922-5338 [Klebsiella michiganensis]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T128729 NZ_CP041514:4922-5338 [Klebsiella michiganensis]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT128729 WP_001261276.1 NZ_CP041514:4695-4925 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT128729 NZ_CP041514:4695-4925 [Klebsiella michiganensis]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATTCGGCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References