Detailed information of TA system TA128613

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54230..54483
• Replicon: plasmid pYPE12-106k
• Accession: NZ_CP041439
• Organism: Escherichia coli strain YPE12

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: FNP80_RS00370
• Protein ID: WP_001312851.1
• Coordinates: 54334..54483 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	54230..54289 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNP80_RS00340	50957..51703	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
FNP80_RS00345	51758..52318	+	561	WP_000139328.1	fertility inhibition protein FinO	-
FNP80_RS00350	52450..52650	+	201	WP_015059022.1	hypothetical protein	-
FNP80_RS00355	53036..53635	+	600	WP_032083981.1	hypothetical protein	-
FNP80_RS00360	53799..54029	+	231	WP_001736714.1	hypothetical protein	-
-	54230..54289	-	60	NuclAT_1	-	Antitoxin
-	54230..54289	-	60	NuclAT_1	-	Antitoxin
-	54230..54289	-	60	NuclAT_1	-	Antitoxin
-	54230..54289	-	60	NuclAT_1	-	Antitoxin
FNP80_RS00370	54334..54483	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
FNP80_RS00375	54767..55015	+	249	WP_000083837.1	replication regulatory protein RepA	-
FNP80_RS00385	55260..55334	+	75	WP_001375168.1	RepA leader peptide Tap	-
FNP80_RS00390	55327..56184	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
FNP80_RS00400	57123..57776	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
FNP80_RS00405	57869..58126	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
FNP80_RS00410	58059..58460	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
FNP80_RS00415	58709..59124	+	416	Protein_71	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / blaTEM-1B / rmtB	-	1..106630	106630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T128613 WP_001312851.1 NZ_CP041439:54334-54483 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T128613 NZ_CP041439:54334-54483 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT128613 NZ_CP041439:c54289-54230 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin