Detailed information of TA system TA128608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 81807..82354
• Replicon: plasmid pSTEC005
• Accession: NZ_CP041438
• Organism: Escherichia coli strain STEC005

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: FNE80_RS24420
• Protein ID: WP_000969996.1
• Coordinates: 82073..82354 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: FNE80_RS24415
• Protein ID: WP_000079941.1
• Coordinates: 81807..82076 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE80_RS24375	77437..77595	+	159	WP_021545194.1	hypothetical protein	-
FNE80_RS24380	77894..78355	+	462	WP_021545193.1	thermonuclease family protein	-
FNE80_RS24385	78401..78610	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
FNE80_RS24390	78648..79238	+	591	WP_032082916.1	DUF2726 domain-containing protein	-
FNE80_RS24395	79478..79735	+	258	WP_000083826.1	replication regulatory protein RepA	-
FNE80_RS24400	79969..80043	+	75	WP_001375168.1	RepA leader peptide Tap	-
FNE80_RS24405	80036..80893	+	858	WP_161620979.1	incFII family plasmid replication initiator RepA	-
FNE80_RS24415	81807..82076	+	270	WP_000079941.1	hypothetical protein	Antitoxin
FNE80_RS24420	82073..82354	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FNE80_RS24425	82618..83790	+	1173	WP_096934598.1	hypothetical protein	-
FNE80_RS24430	83893..84804	+	912	WP_161620980.1	toll/interleukin-1 receptor domain-containing protein	-
FNE80_RS24435	84946..85305	-	360	WP_161620981.1	plasmid partitioning/stability family protein	-
FNE80_RS24440	85340..85891	-	552	Protein_104	plasmid stabilization protein	-
FNE80_RS24445	85885..86106	+	222	WP_077775928.1	hypothetical protein	-
FNE80_RS24450	86128..86463	-	336	WP_001080736.1	colicin transporter	-
FNE80_RS24455	86592..86939	+	348	WP_033812875.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD	1..89435	89435

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T128608 WP_000969996.1 NZ_CP041438:82073-82354 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T128608 NZ_CP041438:82073-82354 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGAACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT128608 WP_000079941.1 NZ_CP041438:81807-82076 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT128608 NZ_CP041438:81807-82076 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A629TXC5