Detailed information of TA system TA128532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3091052..3091889
• Replicon: chromosome
• Accession: NZ_CP041433
• Organism: Escherichia coli strain STEC313

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: FNE82_RS15030
• Protein ID: WP_000227784.1
• Coordinates: 3091052..3091594 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: FNE82_RS15035
• Protein ID: WP_001297137.1
• Coordinates: 3091578..3091889 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE82_RS15010	3086591..3087502	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
FNE82_RS15015	3087670..3088161	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
FNE82_RS15020	3088289..3089653	-	1365	WP_001000974.1	MFS transporter	-
FNE82_RS15025	3090061..3090996	+	936	WP_001365761.1	sel1 repeat family protein	-
FNE82_RS15030	3091052..3091594	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
FNE82_RS15035	3091578..3091889	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
FNE82_RS15040	3092074..3092964	-	891	WP_000971336.1	heme o synthase	-
FNE82_RS15045	3092976..3093305	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
FNE82_RS15050	3093305..3093919	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FNE82_RS15055	3093909..3095900	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FNE82_RS15060	3095922..3096869	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T128532 WP_000227784.1 NZ_CP041433:c3091594-3091052 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T128532 NZ_CP041433:c3091594-3091052 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT128532 WP_001297137.1 NZ_CP041433:c3091889-3091578 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT128532 NZ_CP041433:c3091889-3091578 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3