Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1860350..1860852 Replicon chromosome
Accession NZ_CP041392
Organism Escherichia coli strain ECOL-18-VL-LA-PA-Ryan-0026

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E3Z24_RS08840 Protein ID WP_000767829.1
Coordinates 1860598..1860852 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E3Z24_RS08835 Protein ID WP_001259255.1
Coordinates 1860350..1860601 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3Z24_RS08810 1855527..1856594 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E3Z24_RS08815 1856594..1857664 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
E3Z24_RS08820 1857661..1858965 - 1305 WP_074514295.1 histidinol dehydrogenase -
E3Z24_RS08825 1858971..1859870 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E3Z24_RS08830 1860016..1860066 - 51 WP_001364200.1 his operon leader peptide -
E3Z24_RS08835 1860350..1860601 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E3Z24_RS08840 1860598..1860852 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E3Z24_RS08845 1860935..1861759 + 825 WP_000754737.1 SDR family oxidoreductase -
E3Z24_RS08850 1861805..1862734 + 930 WP_000803366.1 LysR family transcriptional regulator -
E3Z24_RS08855 1862949..1863011 + 63 WP_010723108.1 membrane protein YoeI -
E3Z24_RS08860 1863001..1864359 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E3Z24_RS08865 1864538..1865596 + 1059 WP_087523589.1 transport protein YeeE -
E3Z24_RS08870 1865610..1865837 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128276 WP_000767829.1 NZ_CP041392:1860598-1860852 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128276 NZ_CP041392:1860598-1860852 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128276 WP_001259255.1 NZ_CP041392:1860350-1860601 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128276 NZ_CP041392:1860350-1860601 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References