Detailed information of TA system TA128167

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 88882..89135
• Replicon: plasmid unnamed1
• Accession: NZ_CP041358
• Organism: Escherichia coli strain U1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: E3Z32_RS00635
• Protein ID: WP_001336447.1
• Coordinates: 88986..89135 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	88882..88938 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z32_RS00600	84784..85530	+	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-
E3Z32_RS00605	85585..86142	+	558	WP_000139329.1	fertility inhibition protein FinO	-
E3Z32_RS00610	86294..86497	+	204	WP_001336517.1	hypothetical protein	-
E3Z32_RS00615	87250..87711	+	462	WP_000760080.1	thermonuclease family protein	-
E3Z32_RS00620	87814..88197	+	384	WP_001109264.1	hypothetical protein	-
E3Z32_RS00625	88350..88787	+	438	WP_000872609.1	hypothetical protein	-
-	88882..88938	-	57	NuclAT_1	-	Antitoxin
-	88882..88938	-	57	NuclAT_1	-	Antitoxin
-	88882..88938	-	57	NuclAT_1	-	Antitoxin
-	88882..88938	-	57	NuclAT_1	-	Antitoxin
E3Z32_RS00635	88986..89135	+	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3Z32_RS00640	89420..89677	+	258	WP_000084404.1	replication regulatory protein RepA	-
E3Z32_RS00650	89911..89985	+	75	WP_001365705.1	RepA leader peptide Tap	-
E3Z32_RS00655	89978..90835	+	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
E3Z32_RS00665	91536..91748	+	213	WP_001336932.1	hypothetical protein	-
E3Z32_RS00670	91863..92171	-	309	Protein_114	transposase	-
E3Z32_RS27140	92364..92462	+	99	WP_137491101.1	transposase	-
E3Z32_RS00680	92612..92860	-	249	WP_000738203.1	hypothetical protein	-
E3Z32_RS00685	92890..93660	-	771	WP_000712166.1	molybdopterin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..130003	130003
	-	flank	IS/Tn	-	-	91863..92129	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T128167 WP_001336447.1 NZ_CP041358:88986-89135 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T128167 NZ_CP041358:88986-89135 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT128167 NZ_CP041358:c88938-88882 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin