Detailed information of TA system TA127996

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4067383..4067795
• Replicon: chromosome
• Accession: NZ_CP041302
• Organism: Escherichia coli strain MSHS 472

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: FK543_RS20790
• Protein ID: WP_000132630.1
• Coordinates: 4067454..4067795 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4067383..4067459 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK543_RS20780	4063980..4065449	+	1470	WP_001387312.1	type I restriction-modification system subunit M	-
FK543_RS20785	4065449..4067233	+	1785	WP_074532719.1	restriction endonuclease subunit S	-
-	4067383..4067459	-	77	NuclAT_15	-	Antitoxin
-	4067383..4067459	-	77	NuclAT_15	-	Antitoxin
-	4067383..4067459	-	77	NuclAT_15	-	Antitoxin
-	4067383..4067459	-	77	NuclAT_15	-	Antitoxin
-	4067383..4067459	-	77	NuclAT_16	-	Antitoxin
-	4067383..4067459	-	77	NuclAT_16	-	Antitoxin
-	4067383..4067459	-	77	NuclAT_16	-	Antitoxin
-	4067383..4067459	-	77	NuclAT_16	-	Antitoxin
FK543_RS20790	4067454..4067795	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
FK543_RS20795	4067842..4069935	-	2094	WP_053263422.1	DUF262 domain-containing protein	-
FK543_RS20800	4070032..4070196	-	165	WP_000394276.1	DUF1127 domain-containing protein	-
FK543_RS20805	4070373..4071785	+	1413	WP_000199353.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T127996 WP_000132630.1 NZ_CP041302:4067454-4067795 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T127996 NZ_CP041302:4067454-4067795 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT127996 NZ_CP041302:c4067459-4067383 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin