Detailed information of TA system TA127945

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 190750..191175
• Replicon: plasmid pCys-1
• Accession: NZ_CP041299
• Organism: Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: FK541_RS01080
• Protein ID: WP_001332444.1
• Coordinates: 191014..191175 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	190750..190961 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK541_RS01050	186724..187221	+	498	WP_050427786.1	single-stranded DNA-binding protein	-
FK541_RS01055	187284..187517	+	234	WP_001209608.1	DUF905 domain-containing protein	-
FK541_RS01060	187576..189534	+	1959	Protein_185	ParB/RepB/Spo0J family partition protein	-
FK541_RS01065	189589..190023	+	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
FK541_RS01070	190020..190739	+	720	WP_001276275.1	plasmid SOS inhibition protein A	-
-	190750..190961	+	212	NuclAT_0	-	Antitoxin
-	190750..190961	+	212	NuclAT_0	-	Antitoxin
-	190750..190961	+	212	NuclAT_0	-	Antitoxin
-	190750..190961	+	212	NuclAT_0	-	Antitoxin
FK541_RS01080	191014..191175	+	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
FK541_RS01085	191413..192025	-	613	Protein_189	hypothetical protein	-
FK541_RS01090	192204..193025	+	822	WP_001234485.1	DUF945 domain-containing protein	-
FK541_RS01095	193321..193911	-	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
FK541_RS01105	194246..194629	+	384	WP_000124981.1	relaxosome protein TraM	-
FK541_RS01110	194819..195505	+	687	WP_000332490.1	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucB / iucC / iucD / iutA	1..195702	195702
	-	flank	IS/Tn	-	-	185375..185878	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T127945 WP_001332444.1 NZ_CP041299:191014-191175 [Escherichia coli O1:H42]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T127945 NZ_CP041299:191014-191175 [Escherichia coli O1:H42]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT127945 NZ_CP041299:190750-190961 [Escherichia coli O1:H42]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin