Detailed information of TA system TA127903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 45967..46486
• Replicon: plasmid pVVEswe-R3
• Accession: NZ_CP041264
• Organism: Enterococcus faecium strain VVEswe-R

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: FKW92_RS15640
• Protein ID: WP_000588503.1
• Coordinates: 46229..46486 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: FKW92_RS15635
• Protein ID: WP_000388479.1
• Coordinates: 45967..46236 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FKW92_RS15590	41164..42063	+	900	WP_002303312.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FKW92_RS15595	42066..42551	+	486	WP_002303313.1	single-stranded DNA-binding protein	-
FKW92_RS15600	42692..42895	-	204	WP_002316074.1	putative holin-like toxin	-
FKW92_RS15605	42970..43230	+	261	WP_002295623.1	hypothetical protein	-
FKW92_RS15610	43667..43873	+	207	WP_002347218.1	hypothetical protein	-
FKW92_RS15615	43873..44124	+	252	WP_002296238.1	hypothetical protein	-
FKW92_RS15620	44139..44576	+	438	WP_002300851.1	hypothetical protein	-
FKW92_RS15625	44569..45276	+	708	WP_002303117.1	hypothetical protein	-
FKW92_RS15630	45657..45836	+	180	WP_001120991.1	hypothetical protein	-
FKW92_RS15635	45967..46236	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FKW92_RS15640	46229..46486	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
FKW92_RS15645	46945..47100	+	156	WP_002347142.1	hypothetical protein	-
FKW92_RS15650	47124..47948	+	825	WP_001809248.1	hypothetical protein	-
FKW92_RS15655	48113..48727	-	615	WP_001261742.1	recombinase family protein	-
FKW92_RS15660	48965..49387	+	423	WP_002303116.1	hypothetical protein	-
FKW92_RS15665	49397..49600	+	204	WP_002299573.1	hypothetical protein	-
FKW92_RS15670	49812..50396	+	585	WP_002299575.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..65253	65253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T127903 WP_000588503.1 NZ_CP041264:46229-46486 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T127903 NZ_CP041264:46229-46486 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT127903 WP_000388479.1 NZ_CP041264:45967-46236 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT127903 NZ_CP041264:45967-46236 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7