Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4656934..4657562 Replicon chromosome
Accession NZ_CP041125
Organism Serratia marcescens strain WVU-004

Toxin (Protein)


Gene name higB Uniprot ID A0A221FUH3
Locus tag FG169_RS22380 Protein ID WP_033636092.1
Coordinates 4656934..4657224 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B4GQY7
Locus tag FG169_RS22385 Protein ID WP_015379150.1
Coordinates 4657239..4657562 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FG169_RS22370 4653508..4655529 + 2022 WP_141960314.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
FG169_RS22375 4655564..4656703 - 1140 WP_015379148.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
FG169_RS22380 4656934..4657224 + 291 WP_033636092.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FG169_RS22385 4657239..4657562 + 324 WP_015379150.1 HigA family addiction module antidote protein Antitoxin
FG169_RS22390 4657555..4658067 + 513 WP_016930176.1 M48 family metallopeptidase -
FG169_RS22395 4658118..4659098 + 981 WP_141960316.1 Gfo/Idh/MocA family oxidoreductase -
FG169_RS22400 4659100..4659831 + 732 WP_141960318.1 glutamine amidotransferase -
FG169_RS22405 4660087..4661052 + 966 WP_185741783.1 TerC family protein -
FG169_RS22410 4661315..4662550 + 1236 WP_060430962.1 serine/threonine transporter SstT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(24-71)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11573.23 Da        Isoelectric Point: 10.4937

>T127675 WP_033636092.1 NZ_CP041125:4656934-4657224 [Serratia marcescens]
MIKSFRDRYLEQFYLGGRRSRLIPGALERQLARKLDMLAAAQQERDLHHPTGNYYKRLSGPFQGWSSLRVNMHWRLMFQW
RHDAAEKIYLDPHQDT

Download         Length: 291 bp

>T127675 NZ_CP041125:4656934-4657224 [Serratia marcescens]
ATGATTAAATCTTTCCGCGATCGCTATCTGGAGCAGTTCTATCTGGGAGGCAGGCGCAGCCGTCTTATCCCCGGCGCGCT
CGAGCGGCAGTTGGCCAGAAAGCTCGATATGTTAGCCGCCGCGCAGCAAGAGCGTGATTTGCATCACCCCACGGGTAACT
ATTACAAGCGGCTATCCGGGCCATTTCAGGGATGGTCGAGTCTGCGCGTCAATATGCACTGGCGGCTGATGTTCCAGTGG
CGGCATGATGCCGCCGAAAAAATTTACCTCGATCCTCACCAAGATACCTGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12141.78 Da        Isoelectric Point: 6.2285

>AT127675 WP_015379150.1 NZ_CP041125:4657239-4657562 [Serratia marcescens]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Download         Length: 324 bp

>AT127675 NZ_CP041125:4657239-4657562 [Serratia marcescens]
ATGTTGGAAACCACGACTCGCGAACCCACCACGGTGGGAGAAGTGCTGCAGGAAGAATATTTAACGCCGTTAGGCATCGG
CCAAGAAGAACTGGCAAAGGTATTGGGCGTCACCCGCGCCACCGTCAATCGCCTGTGCAACGGGCATCGACGCATGAGCG
TTGCCGAGGCCAATCTGTTTGCCGATCTGTTCGAAACGACCGCCGACTTTTGGCTAAACCTGCAGGCGGCACACGATCGC
TGGGAAGCCAGACAGGCCGATGCGCAGCGTGTGCGGCTGCGGCCAATCAAAGAAATACTGGGACGCAATATCACTCATGC
ATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A221FUH3

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4GQY7

References