Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 3476226..3476883 Replicon chromosome
Accession NZ_CP041125
Organism Serratia marcescens strain WVU-004

Toxin (Protein)


Gene name higB Uniprot ID A0A0M4RY59
Locus tag FG169_RS16830 Protein ID WP_060420873.1
Coordinates 3476560..3476883 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0M5M3W3
Locus tag FG169_RS16825 Protein ID WP_060420390.1
Coordinates 3476226..3476522 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FG169_RS16805 3472523..3473638 + 1116 WP_100396967.1 glycosyltransferase family 9 protein -
FG169_RS16810 3473562..3474785 - 1224 WP_060439553.1 O-antigen ligase family protein -
FG169_RS16815 3474763..3475671 - 909 WP_060420392.1 hypothetical protein -
FG169_RS16820 3475976..3476218 + 243 WP_025303447.1 hypothetical protein -
FG169_RS16825 3476226..3476522 - 297 WP_060420390.1 helix-turn-helix domain-containing protein Antitoxin
FG169_RS16830 3476560..3476883 - 324 WP_060420873.1 toxin HigB-2 Toxin
FG169_RS16835 3476948..3477577 - 630 WP_141960693.1 alanyl-tRNA editing protein -
FG169_RS16840 3477663..3478598 + 936 WP_060439549.1 LysR family transcriptional regulator -
FG169_RS16845 3478581..3479228 - 648 WP_019452474.1 DNA oxidative demethylase AlkB -
FG169_RS16850 3479368..3480246 + 879 WP_049273981.1 EamA family transporter RarD -
FG169_RS16855 3480248..3481006 - 759 WP_072271000.1 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(45-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-83)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12370.15 Da        Isoelectric Point: 8.8132

>T127669 WP_060420873.1 NZ_CP041125:c3476883-3476560 [Serratia marcescens]
MDYLEFIETTVFSRKRAELMKDDDFQAFQSYLLLNHHEGDTISHTGGCKKIRWGLDGKGKRGGVRVIYYLTAPSGRVYLL
LIYRKNEKDDLSGQEKAILRSIVQQLD

Download         Length: 324 bp

>T127669 NZ_CP041125:c3476883-3476560 [Serratia marcescens]
ATGGACTATCTGGAGTTTATCGAAACGACCGTTTTCAGCCGAAAGCGGGCTGAATTGATGAAGGATGATGACTTTCAGGC
TTTCCAAAGCTATTTGCTGTTGAACCATCATGAAGGGGATACGATCAGCCACACGGGCGGATGCAAAAAGATACGCTGGG
GGCTGGACGGAAAGGGAAAACGCGGCGGCGTAAGAGTGATTTATTATCTGACGGCGCCCAGCGGCCGAGTCTATTTATTG
TTGATATACCGTAAAAATGAAAAGGATGATTTATCCGGGCAGGAAAAGGCGATATTGAGATCCATCGTTCAACAACTTGA
TTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11003.00 Da        Isoelectric Point: 10.1906

>AT127669 WP_060420390.1 NZ_CP041125:c3476522-3476226 [Serratia marcescens]
MKKELFDDLVSSMNEMVAIEKGKMTPKSEHVHRHAIPDVKLLRKSRGMKQAEFAKAVGASVGLVQSWEQERRIPSGSALK
LLLLIERNPALLDELLVI

Download         Length: 297 bp

>AT127669 NZ_CP041125:c3476522-3476226 [Serratia marcescens]
ATGAAAAAAGAACTTTTCGACGATTTGGTCAGCAGTATGAACGAAATGGTGGCGATCGAAAAGGGTAAGATGACGCCAAA
ATCCGAGCATGTTCACCGTCATGCTATTCCAGACGTCAAGCTGCTGCGCAAATCCCGTGGGATGAAGCAGGCGGAGTTTG
CCAAGGCTGTGGGGGCCAGCGTGGGACTGGTGCAAAGCTGGGAGCAGGAACGCCGCATACCCTCGGGGTCGGCACTGAAA
CTTCTGCTGCTTATTGAGCGTAACCCGGCATTGCTCGATGAGTTGCTGGTGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4RY59

Antitoxin

Source ID Structure
AlphaFold DB A0A0M5M3W3

References