Detailed information of TA system TA127620

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29746..30000
• Replicon: plasmid unnamed1
• Accession: NZ_CP041111
• Organism: Escherichia coli strain ECCTRSRTH03

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CI675_RS22775
• Protein ID: WP_001351576.1
• Coordinates: 29746..29952 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	29939..30000 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CI675_RS22735	25638..26342	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CI675_RS22740	26394..26972	+	579	Protein_36	chemotaxis protein	-
CI675_RS22755	28065..28922	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
CI675_RS22760	28915..28989	-	75	WP_061357229.1	RepA leader peptide Tap	-
CI675_RS22770	29221..29478	-	258	WP_001541895.1	replication regulatory protein RepA	-
CI675_RS22775	29746..29952	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	29939..30000	+	62	NuclAT_1	-	Antitoxin
-	29939..30000	+	62	NuclAT_1	-	Antitoxin
-	29939..30000	+	62	NuclAT_1	-	Antitoxin
-	29939..30000	+	62	NuclAT_1	-	Antitoxin
CI675_RS22785	30342..30554	-	213	WP_001541896.1	ANR family transcriptional regulator	-
CI675_RS22790	30689..31249	-	561	WP_000139310.1	fertility inhibition protein FinO	-
CI675_RS22795	31304..32047	-	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA2 / blaCTX-M-55 / mph(A) / erm(B) / aac(3)-IId	-	1..96497	96497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T127620 WP_001351576.1 NZ_CP041111:c29952-29746 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T127620 NZ_CP041111:c29952-29746 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT127620 NZ_CP041111:29939-30000 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin