Detailed information of TA system TA127591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 62408..63051
• Replicon: plasmid unnamed2
• Accession: NZ_CP041094
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPCTRSRTH01

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CI667_RS27000
• Protein ID: WP_094309080.1
• Coordinates: 62635..63051 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6B7Q5S4
• Locus tag: CI667_RS26995
• Protein ID: WP_021312476.1
• Coordinates: 62408..62638 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CI667_RS28855	59218..59504	+	287	Protein_51	hypothetical protein	-
CI667_RS26975	59514..59828	+	315	WP_065801600.1	hypothetical protein	-
CI667_RS26980	59879..61225	-	1347	WP_072123333.1	hypothetical protein	-
CI667_RS26985	61524..62036	+	513	WP_065801590.1	hypothetical protein	-
CI667_RS26995	62408..62638	+	231	WP_021312476.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CI667_RS27000	62635..63051	+	417	WP_094309080.1	type II toxin-antitoxin system VapC family toxin	Toxin
CI667_RS27005	63086..63331	+	246	WP_065801591.1	hypothetical protein	-
CI667_RS27010	63395..63649	+	255	WP_065801592.1	hypothetical protein	-
CI667_RS27020	64206..65212	+	1007	Protein_59	IS110 family transposase	-
CI667_RS27025	65491..65739	+	249	WP_065801593.1	hypothetical protein	-
CI667_RS27030	65744..66316	+	573	WP_094309628.1	hypothetical protein	-
CI667_RS27035	66347..66742	+	396	Protein_62	DNA-binding protein	-
CI667_RS27040	66733..66858	+	126	Protein_63	IS481 family transposase	-
CI667_RS27045	66989..67819	-	831	WP_074425395.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iutA / iucD / iucC / iucB / iucA / cseA	1..168755	168755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14989.47 Da
• Isoelectric Point: 9.2738

>T127591 WP_094309080.1 NZ_CP041094:62635-63051 [Klebsiella pneumoniae subsp. pneumoniae]
VKKTFMLDTKICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T127591 NZ_CP041094:62635-63051 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAAAAAGACTTTTATGCTCGACACAAAAATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCCCCGCGCCATATCGAGCTGGTTGACGCGTTCTGTGCCCGCCTCGATGCCATCCTGCCCTGGGACCGAGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCCTTGCGACAGGCCGGGACGCCGATTGGCCCGAACGACACGGCGATAGC
CGGGCACGCCATCGCGGCCGGCGCCGTTCTGGTGACGAATAATGTAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8723.76 Da
• Isoelectric Point: 4.3410

>AT127591 WP_021312476.1 NZ_CP041094:62408-62638 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT127591 NZ_CP041094:62408-62638 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGCGAGGATGTGGTCAGCGACGAAGGACGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6B7Q5S4