Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1311463..1312099 Replicon chromosome
Accession NZ_CP040931
Organism Bifidobacterium breve strain JR01

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag D3H49_RS05840 Protein ID WP_003829234.1
Coordinates 1311743..1312099 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag D3H49_RS05835 Protein ID WP_012577921.1
Coordinates 1311463..1311756 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3H49_RS05810 1306877..1309555 - 2679 WP_014483804.1 alanine--tRNA ligase -
D3H49_RS05815 1309651..1309887 - 237 WP_003829239.1 hypothetical protein -
D3H49_RS05820 1309887..1310291 - 405 WP_014483805.1 DUF948 domain-containing protein -
D3H49_RS05825 1310374..1311048 - 675 WP_015438748.1 histidine phosphatase family protein -
D3H49_RS05830 1311148..1311312 + 165 Protein_1132 IS30 family transposase -
D3H49_RS05835 1311463..1311756 + 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
D3H49_RS05840 1311743..1312099 + 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D3H49_RS05845 1312276..1312878 - 603 WP_106620803.1 transglutaminase family protein -
D3H49_RS05850 1312983..1313339 - 357 WP_003833231.1 SdpI family protein -
D3H49_RS05855 1313427..1313912 + 486 WP_003829229.1 cytidine deaminase -
D3H49_RS05860 1314052..1314834 + 783 WP_003829227.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
D3H49_RS05865 1315171..1315797 - 627 WP_003829226.1 30S ribosomal protein S4 -
D3H49_RS05870 1315994..1316905 - 912 WP_003829224.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T127302 WP_003829234.1 NZ_CP040931:1311743-1312099 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T127302 NZ_CP040931:1311743-1312099 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT127302 WP_012577921.1 NZ_CP040931:1311463-1311756 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT127302 NZ_CP040931:1311463-1311756 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References