Detailed information of TA system TA127094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 844127..844755
• Replicon: chromosome
• Accession: NZ_CP040840
• Organism: Leptospira weilii strain CUDO6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M6PYD2
• Locus tag: FHG67_RS04075
• Protein ID: WP_004495849.1
• Coordinates: 844354..844755 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FHG67_RS04070
• Protein ID: WP_016759326.1
• Coordinates: 844127..844357 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHG67_RS04020	839183..839875	+	693	WP_004496073.1	cytochrome c biogenesis protein CcsA	-
FHG67_RS04025	839882..841012	+	1131	WP_004499160.1	SpoIIE family protein phosphatase	-
FHG67_RS04030	841009..841875	+	867	WP_016759636.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FHG67_RS04035	841851..842099	+	249	WP_002614445.1	phosphoribosylformylglycinamidine synthase subunit PurS	-
FHG67_RS04040	842096..842755	+	660	WP_002614451.1	phosphoribosylformylglycinamidine synthase subunit PurQ	-
FHG67_RS04045	842872..843090	-	219	WP_004499270.1	IS982 family transposase	-
FHG67_RS04050	843091..843197	-	107	Protein_737	IS982 family transposase	-
FHG67_RS21640	843299..843882	-	584	Protein_738	IS982 family transposase	-
FHG67_RS04070	844127..844357	+	231	WP_016759326.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FHG67_RS04075	844354..844755	+	402	WP_004495849.1	type II toxin-antitoxin system VapC family toxin	Toxin
FHG67_RS04080	845041..846198	+	1158	WP_036075452.1	hypothetical protein	-
FHG67_RS04085	846202..847032	+	831	WP_004501498.1	hypothetical protein	-
FHG67_RS04090	847186..847710	+	525	WP_004499241.1	hypothetical protein	-
FHG67_RS04100	848469..849188	+	720	WP_142499657.1	endoflagellar filament sheath protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15600.27 Da
• Isoelectric Point: 9.2916

>T127094 WP_004495849.1 NZ_CP040840:844354-844755 [Leptospira weilii]
MTQYLLDTNICIYIINQKPESVYKKFKKIKLENIFISSITEFELKYGVQKSLHFERNLKVLEEFLSYLNILPFVSEDTNK
AAKVRVELERVGKPIGPFDLLIASQALSNKLTLVTNNEKEFTRIKEIKIENWL

Nucleotide

• Length: 402 bp

>T127094 NZ_CP040840:844354-844755 [Leptospira weilii]
ATGACTCAATACTTGTTGGATACGAATATCTGTATCTATATAATTAATCAAAAGCCGGAATCTGTTTATAAGAAATTTAA
AAAGATTAAATTAGAGAATATTTTCATTTCATCAATTACGGAATTTGAATTAAAATACGGTGTTCAGAAGAGTCTTCATT
TTGAAAGAAATCTGAAAGTCTTAGAAGAATTTTTAAGTTATTTGAATATTCTTCCGTTTGTTAGTGAAGATACGAATAAA
GCTGCAAAAGTTCGAGTAGAATTAGAAAGAGTAGGGAAACCAATTGGTCCTTTCGATCTTCTAATCGCTTCTCAGGCATT
ATCGAATAAACTTACGCTTGTAACAAACAATGAAAAAGAATTTACTCGGATTAAAGAAATTAAAATTGAAAACTGGCTTT
AA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8975.12 Da
• Isoelectric Point: 9.8802

>AT127094 WP_016759326.1 NZ_CP040840:844127-844357 [Leptospira weilii]
MNKAKIFKNGDSQAIRLPKDYRFKGKEVYIRKDGDNVILTPIDDAVDRFWNTVNNFSEDLLIDRNQPNGYDKRNRI

Nucleotide

• Length: 231 bp

>AT127094 NZ_CP040840:844127-844357 [Leptospira weilii]
ATGAATAAAGCTAAAATTTTTAAAAATGGCGACAGCCAAGCAATACGTTTACCAAAGGACTATCGTTTTAAGGGTAAAGA
AGTTTATATACGAAAAGATGGCGATAACGTCATTTTAACGCCAATAGACGATGCGGTTGATCGATTTTGGAATACGGTTA
ATAATTTTTCTGAAGATCTGCTTATTGATCGGAATCAACCAAATGGCTACGATAAACGAAATAGAATATGA

Structures

Toxin

• AlphaFold DB: A0A828Z772